Zhao Xing-cun, Gu Jin-bao, Li Hua, Liu Min, Shen Hao-xian, Chen Xiao-guang
Department of Pathogenic Biology, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Mar;26(3):284-6, 289.
To identify antigens which may help evaluate the therapeutic effect of angiostrongyliasis from adult worm antigen of Angiostrongylus cantonensis.
The adult worm antigens of A. cantonensis were analyzed by Western blotting with the sera of rats infected with A. cantonensis before and after treatment. The sera of rats were tested by enzyme-linked immunosorbent assay (ELISA).
The antigens with relative molecular mass between 38,000 and 78,000 reacted not only with the sera of rats before treatment, but also with that after treatment. The antigens with M(r) between 190,000 and 17,000 reacted with the sera of rats before treatment but not with that after treatment; those with M(r) between 32,000 and 24,000 antigens strongly reacted with the former, but the reaction became much weakened with the latter. The AC32-IgG antibody appeared earlier than the AC-IgG, and disappeared rapidly after treatment. Six of the 10 treated rats became negative for AC-IgG as found by ELISA.
The antigens of adult worm antigen of A. cantonensis with M(r) of 190,000, 32,000, 24,000, 17,000 and 16,000 may serve as candidate antigens for therapeutic effect evaluation of angiostrongyliasis.
从广州管圆线虫成虫抗原中鉴定出有助于评估管圆线虫病治疗效果的抗原。
用感染广州管圆线虫前后大鼠的血清，通过蛋白质印迹法分析广州管圆线虫成虫抗原。用酶联免疫吸附测定法（ELISA）检测大鼠血清。
相对分子质量在38,000至78,000之间的抗原不仅与治疗前大鼠血清反应，也与治疗后大鼠血清反应。相对分子质量在190,000至17,000之间的抗原与治疗前大鼠血清反应，但与治疗后大鼠血清不反应；相对分子质量在32,000至24,000之间的抗原与前者强烈反应，但与后者反应明显减弱。AC32-IgG抗体比AC-IgG出现早，治疗后迅速消失。ELISA检测发现，10只治疗大鼠中有6只AC-IgG呈阴性。
相对分子质量为190,000、32,000、24,000、17,000和16,000的广州管圆线虫成虫抗原可作为评估管圆线虫病治疗效果的候选抗原。
Zotero 插件