Fernandez-Gonzalez Rodrigo, Barcellos-Hoff Mary Helen, Ortiz-de-Solórzano Carlos
Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, California, USA.
J Mammary Gland Biol Neoplasia. 2004 Oct;9(4):343-59. doi: 10.1007/s10911-004-1405-9.
In this paper we present a summary of recent quantitative approaches used for the analysis of macro and microscopic images in mammary gland biology. The advantages and disadvantages of whole mount analysis, reconstruction of serial tissue sections and nucleus/cell segmentation of either conventional and confocal images are discussed, as are applications of quantitative image analysis, such as quantification of protein levels or vasculature measurements in normal tissue and cancer. Integration of quantitative imaging into the further study of the mammary gland holds the promise of better understanding its tissue complexity that evolves during development, differentiation and disease.
在本文中，我们总结了近期用于乳腺生物学中宏观和微观图像分析的定量方法。讨论了整体标本分析、连续组织切片重建以及传统图像和共聚焦图像的细胞核/细胞分割的优缺点，还讨论了定量图像分析的应用，如正常组织和癌症中蛋白质水平的定量或血管系统测量。将定量成像整合到乳腺的进一步研究中，有望更好地理解其在发育、分化和疾病过程中演变的组织复杂性。
