Sho Kenichiro, Takahashi Kanji, Fukuchi Toshio, Matsumura Miyo
Department of Ophthalmology, Kansai Medical University, Osaka, Japan.
Jpn J Ophthalmol. 2005 Mar-Apr;49(2):109-13. doi: 10.1007/s10384-004-0150-3.
To determine whether optical coherence tomography (OCT) can detect early retinal changes after ischemia-reperfusion injury in rats.
The intraocular pressure (IOP) was elevated to induce retinal ischemia in brown Norway rats. After 90 min of ischemia, the IOP was reduced, and after reperfusion of 1, 2, 4, or 7 days, OCT was performed. After the OCT examination, the eyes were enucleated and histological sections were made.
The OCT-determined mean retinal thickness was 168 +/- 16.9 microm in the untreated control group, and 177 +/- 2.16, 170 +/- 7.55, 159 +/- 5.34, and 140 +/- 5.56 microm on days 1, 2, 4, and 7, respectively, in the ischemia-reperfusion group. The histologically determined retinal thicknesses correlated with those obtained by tomographic images, but the histologic thicknesses were 9.5% to 18.5% thinner than those obtained by OCT. Fixation and dehydration of the histological specimens most likely caused tissue shrinkage.
OCT can detect retinal changes quantitatively after ischemia-reperfusion injury, and the retinal thicknesses obtained from OCT images are probably a better measure of the true retinal thickness than those measured on histological sections.
确定光学相干断层扫描（OCT）能否检测大鼠缺血再灌注损伤后的早期视网膜变化。
升高棕色挪威大鼠的眼压以诱导视网膜缺血。缺血90分钟后降低眼压，并在再灌注1、2、4或7天后进行OCT检查。OCT检查后，摘除眼球并制作组织切片。
未治疗的对照组经OCT测定的平均视网膜厚度为168±16.9微米，缺血再灌注组在第1、2、4和7天的平均视网膜厚度分别为177±2.16、170±7.55、159±5.34和140±5.56微米。组织学测定的视网膜厚度与断层图像获得的厚度相关，但组织学厚度比OCT获得的厚度薄9.5%至18.5%。组织学标本的固定和脱水很可能导致组织收缩。
OCT能够定量检测缺血再灌注损伤后的视网膜变化，并且从OCT图像获得的视网膜厚度可能比组织切片测量的更能准确反映真实的视网膜厚度。
