Akinremi Titilola O, Nazeer Saloney, Tötsch Martin
Department of Pathology, Federal Medical Centre, Abeokuta, Nigeria.
Acta Cytol. 2005 Mar-Apr;49(2):169-72. doi: 10.1159/000326127.
To describe a low-cost Papanicolaou staining procedure that can be applied to conventional and monolayer gynecologic preparations.
The amount of alcohol consumed in the procedure, which normally accounts for > 80% of the cost of processing, was reduced drastically by (1) using only 1 modified cytoplasmic counterstain (EA type), thereby (2) reducing the number of alcohol rinses by over half Orange-G dye is omitted.
The resultant effect of the modified staining protocol is quite satisfactory and attractive to screening eyes: nuclear details are sharp and crisp, while the cytoplasm contains transparent differential staining with blue-green and pink.
A reduction in the cost of staining should encourage cervical cancer screening, especially in developing countries, where cost is a limiting factor, thus making it possible for more women to be screened without increasing the cost of the program.
描述一种可应用于传统涂片和单层妇科标本的低成本巴氏染色程序。
该程序中消耗的酒精量通常占处理成本的80%以上，通过以下方式大幅降低酒精用量：（1）仅使用1种改良的细胞质复染剂（EA型），从而（2）将酒精冲洗次数减少一半以上，省略橘黄G染料。
改良染色方案的最终效果令人十分满意，对筛查人员很有吸引力：细胞核细节清晰锐利，而细胞质呈现出蓝绿色和粉红色的透明差异染色。
染色成本的降低应能促进宫颈癌筛查，尤其是在成本是限制因素的发展中国家，从而使更多女性能够接受筛查，而无需增加项目成本。
