Gao Min, Cao Qian, Luo Ling-he, Wu Min-liang, Hu Wei-ling, Si Jian-min
GI Division of Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
Zhonghua Nei Ke Za Zhi. 2005 Mar;44(3):210-2.
Previous studies have shown NOD2/CARD15 gene is the first susceptibility gene to Crohn's disease (CD), three single nucleotide polymorphisms (SNPs) of the gene have been identified to be associated with CD in the Caucasians, but not in the Japanese. Here we have evaluated the NOD2/CARD gene polymorphisms in Chinese patients to determine whether the gene is associated with susceptibility to CD in Chinese Han population.
Blood samples were obtained from 32 patients with CD, 110 patients with ulcerative colitis, and 292 healthy controls in Zhejiang location. Genotyping for 3 common NOD2/CARD15 (Arg702Trp, Gly908Arg, Leu1007fsinsC) polymorphisms was carried out using polymerase chain sequence with specific primer.
None of the patients with CD had heterozygous or homozygous SNPs variants. Similarly none of the ulcerative colitis or health controls.
The common variants in NOD2/CARD15 found in Caucasians with CD are not associated with CD in the Chinese Han population.
既往研究表明，NOD2/CARD15基因是克罗恩病（CD）的首个易感基因，该基因的三个单核苷酸多态性（SNP）已被确定与白种人中的CD相关，但在日本人中并非如此。在此，我们评估了中国患者中NOD2/CARD基因多态性，以确定该基因是否与中国汉族人群对CD的易感性相关。
采集浙江地区32例CD患者、110例溃疡性结肠炎患者及292例健康对照者的血样。采用特异性引物聚合酶链序列对3种常见的NOD2/CARD15（Arg702Trp、Gly908Arg、Leu1007fsinsC）多态性进行基因分型。
CD患者中均无杂合或纯合SNP变异。同样，溃疡性结肠炎患者或健康对照者中也均无。
在患有CD的白种人中发现的NOD2/CARD15常见变异与中国汉族人群的CD无关。
