[中国北方妊娠期高血压疾病患者β2肾上腺素能受体基因单核苷酸多态性研究]

[Study on single nucleotide polymorphism of beta2 adrenoceptor gene in patients with pregnancy induced hypertension of northern China].

作者信息

Fan Li-mei, Zhang Wei-yuan, Zhao Yan-hui, Shi Jie-ping

机构信息

Department of Obstetrics and Gynecology, Second Hospital, Jilin University, Changchun 130041, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2005 Feb;40(2):103-5.

PMID:15840289

Abstract

OBJECTIVE

To study the association of single nucleotide polymorphism (SNP) at locus +46 of human beta(2)-adrenoceptor (beta(2)-AR) gene with pregnancy induced hypertension (PIH) in women from northern China.

METHOD

One hundred and eight women with PIH (PIH group) and ninety seven normal pregnant women (control group) were selected. SNP at locus +46 of human beta(2)-AR was genotyped using PCR-based restriction fragment length polymorphism (RFLP) method.

RESULTS

(1) The frequencies of AA, AG and GG genotypes beta(2)-AR at locus +46 were 24.1%, 25.0% and 50.9% in PIH group, respectively. They were 24.7%, 29.9%, and 45.4% in control group, respectively (P > 0.05). The frequencies of the two alleles A and G were 36.6%, 63.4% in PIH group, and 39.7% and 60.3% in control group, respectively (P > 0.05). (2) There was a significant difference in the distribution frequency of beta(2)-AR at locus +46 between mild, moderate and severe degrees of PIH (P < 0.05).

CONCLUSION

It indicates that, SNP at locus +46 of beta(2)-AR gene is not associated with generation of PIH in northern China, but it has an association with the severity of PIH.

摘要

目的

研究中国北方女性人群中人类β₂-肾上腺素能受体（β₂-AR）基因+46位点单核苷酸多态性（SNP）与妊娠高血压综合征（PIH）的关系。

方法

选取108例PIH患者（PIH组）和97例正常孕妇（对照组）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对人类β₂-AR基因+46位点进行SNP基因分型。

结果

（1）PIH组β₂-AR基因+46位点AA、AG和GG基因型频率分别为24.1%、25.0%和50.9%。对照组分别为24.7%、29.9%和45.4%（P>0.05）。PIH组A、G两个等位基因频率分别为36.6%、63.4%，对照组分别为39.7%和60.3%（P>0.05）。（2）轻度、中度和重度PIH患者β₂-AR基因+46位点分布频率差异有统计学意义（P<0.05）。