Li Wen, Peng Zhi-lan, Cao Ze-yi
Department of Obstetrics and Gynecology, 452 Hospital of People's Liberation Army, Chengdu, China.
Zhonghua Fu Chan Ke Za Zhi. 2005 Mar;40(3):186-9.
To study the effect of thalidomide (Thd) used alone and in combination with cytoxan (CTX) on the growth and angiogenesis of human ovarian cancer transplanted subcutaneously in nude mice.
Human ovarian cancer model transplanted subcutaneously in nude mice was established, and divided into 3 groups: control group, Thd group, and Thd + CTX group. Tumor volume and weight, vascular endothelial growth factor (VEGF) mRNA, VEGF protein, microvascular density (MVD) were detected. The level of VEGF mRNA in tumor tissue was determined by relative quantative reverse transcription polymerase chain reaction. VEGF protein level in serum was determined by enzyme-linked immunosorbent assay (ELISA). MVD was calculated by immunohistochemistry.
(1) Tumor volumes in Thd group and Thd + CTX group were smaller than those in control group (P < 0.05). (2) Expression of VEGF mRNA level in Thd group (55 +/- 9) and Thd + CTX group (26 +/- 7) was significantly lower than that in control group (79 +/- 7, P < 0.01). Serum VEGF level in Thd group, [(29 +/- 10) pg/ml] and Thd + CTX group [(12 +/- 6) pg/ml] was significantly lower than that in control group, [(71 +/- 16) pg/ml, P < 0.01]. (3) MVD in Thd group (12.6 +/- 3.3) and Thd + CTX group (10.6 +/- 1.9) was significantly smaller than that in control group (19.3 +/- 2.8, P < 0.01).
Thalidomide can inhibit the growth and angiogenesis of human ovarian cancer transplanted subcutaneously in nude mice. Treatment with thalidomide is a potentially useful antitumor therapy for ovarian cancer.
研究沙利度胺（Thd）单独及与环磷酰胺（CTX）联合应用对人卵巢癌裸鼠皮下移植瘤生长及血管生成的影响。
建立人卵巢癌裸鼠皮下移植瘤模型，分为3组：对照组、Thd组、Thd + CTX组。检测肿瘤体积和重量、血管内皮生长因子（VEGF）mRNA、VEGF蛋白、微血管密度（MVD）。采用相对定量逆转录聚合酶链反应检测肿瘤组织中VEGF mRNA水平。采用酶联免疫吸附测定（ELISA）法检测血清中VEGF蛋白水平。通过免疫组化计算MVD。
（1）Thd组和Thd + CTX组肿瘤体积小于对照组（P < 0.05）。（2）Thd组（55 ± 9）和Thd + CTX组（26 ± 7）VEGF mRNA水平表达明显低于对照组（79 ± 7，P < 0.01）。Thd组血清VEGF水平为[（29 ± 10）pg/ml]，Thd + CTX组为[（12 ± 6）pg/ml]，明显低于对照组[（71 ± 16）pg/ml，P < 0.01]。（3）Thd组（12.6 ± 3.3）和Thd + CTX组（10.6 ± 1.9）的MVD明显小于对照组（19.3 ± 2.8，P < 0.01）。
沙利度胺可抑制人卵巢癌裸鼠皮下移植瘤的生长及血管生成。沙利度胺治疗可能是一种有效的卵巢癌抗肿瘤治疗方法。
