Thorsteinsson Marc V, Richter Jesse, Lee Ann L, DePhillips Peter
Department of Biologics Development and Engineering, Merck Research Labs, Merck & Co., Inc., West Point, PA 19486, USA.
Anal Biochem. 2005 May 15;340(2):220-5. doi: 10.1016/j.ab.2005.01.006.
A method for determining the critical micelle concentration (CMC) of various detergents based on fluorescence polarization (anisotropy) of the lipophilic probe 5-dodecanoylaminofluorescein is presented. Nonionic, cationic, anionic, and steroid-based detergents can all be evaluated by this method and the determined CMC values of selected detergents agree well with those reported in the literature. In addition, we report the CMC of domiphen bromide, whose CMC value has not previously been described. In the case of ionic detergents, the method described is particularly sensitive at discerning changes in the CMC with increasing ionic strength of the medium and can discriminate detergent CMCs in 5 mM versus 25 mM buffering components. The described fluorescence polarization technique allows very low (submicromolar) concentrations of probe to be employed, thus minimizing the perturbation of micelle formation by 5-dodecanoylaminofluorescein insertion.
本文介绍了一种基于亲脂性探针5-十二烷酰氨基荧光素的荧光偏振（各向异性）来测定各种洗涤剂临界胶束浓度（CMC）的方法。非离子型、阳离子型、阴离子型和类固醇基洗涤剂均可通过该方法进行评估，所选洗涤剂的测定CMC值与文献报道的值吻合良好。此外，我们还报告了度米芬溴化物的CMC，其CMC值此前未曾有过描述。对于离子型洗涤剂，所描述的方法在识别随着介质离子强度增加而引起的CMC变化时特别灵敏，并且能够区分5 mM与25 mM缓冲成分中的洗涤剂CMC。所描述的荧光偏振技术允许使用极低（亚微摩尔）浓度的探针，从而将5-十二烷酰氨基荧光素插入对胶束形成的扰动降至最低。
