Saitoh A, Tanaka N, Nakata A, Ikuta K, Shinagawa H
Department of Experimental Chemotherapy, Osaka University, Japan.
Microbiol Immunol. 1992;36(1):105-11. doi: 10.1111/j.1348-0421.1992.tb01647.x.
The entire and partial gag regions of human immunodeficiency virus type 1 (HIV-1) were overproduced in Escherichia coli and used for epitope mapping of antibodies against p17. We found that a mouse monoclonal antibody to p17, V17 recognizes the mature p17 but not the unprocessed Gag proteins containing the entire p17 moiety. Further analysis revealed that V17 recognizes the C-terminal 12-amino-acid region of p17 having free C-terminus. This monoclonal antibody may be useful for monitoring the maturation of virus particles.
1型人类免疫缺陷病毒（HIV-1）的完整和部分gag区域在大肠杆菌中过量表达，并用于针对p17抗体的表位作图。我们发现，一种针对p17的小鼠单克隆抗体V17识别成熟的p17，但不识别包含整个p17部分的未加工的Gag蛋白。进一步分析表明，V17识别具有游离C末端的p17的C末端12个氨基酸区域。这种单克隆抗体可能有助于监测病毒颗粒的成熟。
