Fréchette J-P, Martineau I, Gagnon G
Faculté de médecine dentaire, Université Laval, Québec, Province Québec, Canada G1K 7P4.
J Dent Res. 2005 May;84(5):434-9. doi: 10.1177/154405910508400507.
Platelet-rich plasmas (PRPs) are used in a variety of clinical applications, based on the premise that higher growth factor content should promote better healing. In this study, we have determined the effects of calcium and thrombin on the release of EGF, TGF-alpha, IGF-1, Ang-2 and IL-1beta from PRPs, and assessed the mitogenic potential of PRP supernatants on osteoblast and endothelial cell division. ELISA assays indicate that (i) mean growth factor concentrations vary from traces (TGF-alpha) to 5.5 ng/mL (IGF-1), (ii) there are significant variations in growth factor concentrations between individuals, and (iii) calcium and thrombin regulate growth factor release, synthesis, and/or degradation in stereotyped patterns that are specific to each growth factor. PRP supernatants promote strong osteoblast and endothelial cell divisions, supporting the concept that PRPs may be beneficial in wound healing.
PRPs, platelet-rich plasmas; GFs, growth factors; EGF, epidermal growth factor; TGF-alpha, transforming growth factor-alpha; IGF-1, insulin-like growth factor-1; Ang-2, angiopoietin-2; IL-1beta, interleukin-1 beta; HUVECs, human umbilical vein endothelial cells; hFOB 1.19, human fetal osteoblasts; and FBS, fetal bovine serum.
富血小板血浆（PRP）基于更高的生长因子含量应促进更好愈合的前提，被用于多种临床应用。在本研究中，我们已确定钙和凝血酶对PRP中表皮生长因子（EGF）、转化生长因子-α（TGF-α）、胰岛素样生长因子-1（IGF-1）、血管生成素-2（Ang-2）和白细胞介素-1β（IL-1β）释放的影响，并评估了PRP上清液对成骨细胞和内皮细胞分裂的促有丝分裂潜力。酶联免疫吸附测定表明：（i）生长因子平均浓度从痕量（TGF-α）到5.5纳克/毫升（IGF-1）不等；（ii）个体间生长因子浓度存在显著差异；（iii）钙和凝血酶以特定于每种生长因子的固定模式调节生长因子的释放、合成和/或降解。PRP上清液促进强烈的成骨细胞和内皮细胞分裂，支持了PRP可能对伤口愈合有益的观点。
PRP，富血小板血浆；GF，生长因子；EGF，表皮生长因子；TGF-α，转化生长因子-α；IGF-1，胰岛素样生长因子-1；Ang-2，血管生成素-2；IL-1β，白细胞介素-1β；HUVEC，人脐静脉内皮细胞；hFOB 1.19，人胎儿成骨细胞；FBS，胎牛血清。
