Nave Colin, Garman Elspeth F
CCLRC Daresbury Laboratory, Warrington, UK.
J Synchrotron Radiat. 2005 May;12(Pt 3):257-60. doi: 10.1107/S0909049505007132. Epub 2005 Apr 14.
Interest in radiation damage is growing rapidly owing to the surge in macromolecular crystallography experiments carried out at modern brilliant synchrotron macromolecular crystallography beamlines. Work on the characterization of radiation damage in cryocooled protein crystals is starting to have some impact on our understanding of the problem and of how damage might be affecting both the process of structure solution and the actual structure obtained. A brief review of the most recent developments is given together with an assessment of the remaining problems. Although progress is being made, the understanding of radiation damage is far from complete. Methods for recognizing the damage and treating the data are being made available but they are still at an early stage of development.
由于在现代高亮度同步加速器大分子晶体学光束线上进行的大分子晶体学实验激增，对辐射损伤的关注正在迅速增长。关于低温冷却蛋白质晶体中辐射损伤特征的研究开始对我们理解这一问题以及损伤如何影响结构解析过程和所获得的实际结构产生了一些影响。本文简要回顾了最新进展，并对遗留问题进行了评估。尽管取得了进展，但对辐射损伤的理解还远未完善。识别损伤和处理数据的方法正在出现，但仍处于早期发展阶段。
