Garman Elspeth F, Owen Robin Leslie
Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, England.
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):32-47. doi: 10.1107/S0907444905034207. Epub 2005 Dec 14.
Advances in cryocrystallographic techniques for macromolecular crystallography have been intimately intertwined with efforts to reduce the deleterious effects of X-ray damage inflicted during the collection of diffraction data. A brief overview of cryomethods and their rationale is given. This is followed by a summary of our current limited understanding of radiation damage in cryocooled crystals, investigations aimed at minimizing its effects and finally some developments which actually utilize it both for phasing and to extend structural knowledge.
用于大分子晶体学的低温晶体学技术的进展与减少衍射数据收集过程中X射线损伤的有害影响的努力紧密相连。本文简要概述了低温方法及其原理。随后总结了我们目前对低温冷却晶体中辐射损伤的有限理解、旨在最小化其影响的研究，最后介绍了一些实际利用辐射损伤进行相位确定和扩展结构知识的进展。
