Fogdell-Hahn A, Soldan S S, Shue S, Akhyani N, Refai H, Ahlqvist J, Jacobson S
Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke (NINDS), National Institutes of Health (NIH), 9000 Rockville Pike, Building 10, Room 5B-16, Bethesda, MD 20892, USA.
Virus Res. 2005 Jun;110(1-2):57-63. doi: 10.1016/j.virusres.2005.01.005.
The association of human herpesvirus 6 (HHV-6) and multiple sclerosis (MS) has been supported by several immunological and molecular studies. Recently, membrane cofactor protein (CD46) has been identified as the cellular receptor for the A and B variants of HHV-6. Elevated levels of soluble CD46 (sCD46) have been reported in the serum and CSF of MS patients. The aim of this study was to investigate a possible correlation between elevated levels of soluble CD46 and the presence of serum HHV-6 DNA in MS patients. An immunoaffinity column comprised of immobilized monoclonal antibodies to CD46 was developed to isolate sCD46 from cell free body fluids of MS patients and controls. After immunoaffinity purification, DNA was extracted from anti-CD46 column eluates and subjected to PCR amplification. Of the 42 MS samples tested, 4 serum samples were HHV-6 positive, 3 of which were typed as HHV-6A. The co-purification of sCD46 and HHV-6 DNA from MS sera indicates that HHV-6 is tightly connected to its receptor, CD46, in the serum of MS patients.
多项免疫学和分子研究支持了人类疱疹病毒6型（HHV-6）与多发性硬化症（MS）之间的关联。最近，膜辅因子蛋白（CD46）已被确定为HHV-6 A和B变体的细胞受体。据报道，MS患者的血清和脑脊液中可溶性CD46（sCD46）水平升高。本研究的目的是调查MS患者中可溶性CD46水平升高与血清HHV-6 DNA存在之间可能的相关性。开发了一种由固定化抗CD46单克隆抗体组成的免疫亲和柱，用于从MS患者和对照的无细胞体液中分离sCD46。免疫亲和纯化后，从抗CD46柱洗脱液中提取DNA并进行PCR扩增。在测试的42份MS样本中，4份血清样本HHV-6呈阳性，其中3份被鉴定为HHV-6A。从MS血清中共纯化sCD46和HHV-6 DNA表明，在MS患者血清中，HHV-6与其受体CD46紧密相连。
