Rameshthangam P, Ramasamy P
Department of Biotechnology, Life Sciences Building, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.
Virus Res. 2005 Jun;110(1-2):133-41. doi: 10.1016/j.virusres.2005.02.003.
White spot syndrome virus (WSSV) is the causative agent of the white spot disease of shrimp. Penaeus monodon were captured from Muttukadu Estuary in Chennai, India, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. WSSV-free P. monodon were challenged by feeding them only once with WSSV-infected tissues of P. monodon. Cumulative mortality (100%) of the infected individuals was determined. Tissues from infected and uninfected shrimp such as muscles, hepatopancreas, heart, gills and eye tissues (100mg of each) and haemolymph (50 microl) were subjected to SDS-PAGE. In infected muscle tissue, six newly expressed proteins were detected. In infected haemolymph, four new proteins and three intensely expressed high molecular weight proteins were observed. Three intensely expressed high molecular weight proteins were detected in infected heart tissue and two new proteins in infected hepatopancreatic tissues. In infected gill tissues, two new protein bands and three intensely expressed high molecular weight proteins were detected on comparison with uninfected tissues. Similarly, two intensely expressed protein bands were seen in the infected as compared with the uninfected eye tissues. The protein profiles of the muscle tissue from 50 different naturally infected (WSSV) shrimp were analyzed. Eleven different new protein bands appeared in the infected muscle tissues when compared to the control muscle tissues whereas; in muscle tissue six new proteins were observed both in naturally and experimentally WSSV infected shrimp. The current study has shown that the protein expression patterns of the infected tissues of P. monodon have been drastically altered by WSSV infection. Western blot analysis revealed that one of the newly expressed 53 kDa protein in the infected muscle represents the WSSV envelope protein.
白斑综合征病毒（WSSV）是对虾白斑病的病原体。斑节对虾从印度钦奈的穆图卡杜河口捕获，运输到实验室并饲养在具有连续水循环 - 生物过滤的曝气系统中。无WSSV的斑节对虾仅通过喂食一次WSSV感染的斑节对虾组织进行攻毒。测定感染个体的累计死亡率（100%）。将感染和未感染虾的组织，如肌肉、肝胰腺、心脏、鳃和眼组织（各100mg）以及血淋巴（50微升）进行SDS - PAGE分析。在感染的肌肉组织中，检测到六种新表达的蛋白质。在感染的血淋巴中，观察到四种新蛋白质和三种高表达的高分子量蛋白质。在感染的心脏组织中检测到三种高表达的高分子量蛋白质，在感染的肝胰腺组织中检测到两种新蛋白质。与未感染组织相比，在感染的鳃组织中检测到两条新蛋白质条带和三种高表达的高分子量蛋白质。同样，与未感染的眼组织相比，在感染的眼组织中观察到两条高表达的蛋白质条带。分析了50只不同的自然感染（WSSV）虾的肌肉组织的蛋白质谱。与对照肌肉组织相比，感染的肌肉组织中出现了11条不同的新蛋白质条带；而在自然感染和实验感染WSSV的虾的肌肉组织中均观察到六种新蛋白质。当前研究表明，WSSV感染极大地改变了斑节对虾感染组织的蛋白质表达模式。蛋白质印迹分析显示，感染肌肉中新表达的53 kDa蛋白质之一代表WSSV包膜蛋白。
