Vianna M C B, Sreekumar C, Miska K B, Hill D E, Dubey J P
United States Department of Agriculture, Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, Beltsville Agricultural Research Center, Building 1001, Beltsville, MD 20705, USA.
Vet Parasitol. 2005 May 15;129(3-4):253-7. doi: 10.1016/j.vetpar.2004.02.031.
Attempts were made to isolate Neospora caninum from naturally infected white-tailed deer (Odocoileus virginianus). A total of 110 deer killed during the 2003 hunting season in Virginia region were used for the isolation of N. caninum. Of these, brains from 28 deer that had NAT titer of 1:200 were inoculated into interferon-gamma gene knock out (KO) mice. N. caninum was isolated from the tissues of three deer and all three isolates were mildly virulent to KO mice. Only one of the isolates could be adapted to in vitro growth. Protozoa in the tissues of KO mice reacted with N. caninum-specific polyclonal antibodies and N. caninum DNA was demonstrated in infected tissues by PCR assays; sequences of portions of the ITS-1 and gene 5 loci were identical to those in the public database. This is the first record of in vitro isolation of N. caninum from white-tailed deer and lends credence to the white-tailed deer as an intermediate host for this parasite.
研究人员尝试从自然感染的白尾鹿（弗吉尼亚鹿）中分离犬新孢子虫。2003年弗吉尼亚地区狩猎季节捕杀的110只鹿被用于犬新孢子虫的分离。其中，将28只NAT效价为1:200的鹿的脑组织接种到干扰素-γ基因敲除（KO）小鼠体内。从三只鹿的组织中分离出了犬新孢子虫，且所有三个分离株对KO小鼠的毒力均较弱。只有一个分离株能够适应体外生长。KO小鼠组织中的原生动物与犬新孢子虫特异性多克隆抗体发生反应，并且通过PCR检测在感染组织中证实了犬新孢子虫DNA的存在；ITS-1和基因5位点部分序列与公共数据库中的序列相同。这是首次从白尾鹿中体外分离出犬新孢子虫的记录，证实了白尾鹿是这种寄生虫的中间宿主。
