通过表达编码生物膜胞外多糖产生的ica基因座，表皮葡萄球菌菌株从共生菌转变为侵袭性菌。
Conversion of Staphylococcus epidermidis strains from commensal to invasive by expression of the ica locus encoding production of biofilm exopolysaccharide.
作者信息
Li Hualin, Xu Lin, Wang Jianping, Wen Yumei, Vuong Cuong, Otto Michael, Gao Qian
机构信息
Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, 200032, PR China.
出版信息
Infect Immun. 2005 May;73(5):3188-91. doi: 10.1128/IAI.73.5.3188-3191.2005.
Abstract
To test if biofilm formation in Staphylococcus epidermidis is dependent on the polysaccharide intercellular adhesin, whose biosynthesis is driven by the ica locus, a plasmid containing the ica locus was transferred to three ica-negative strains. Using in vitro biofilm assays and a rat central venous catheter infection model, we confirmed the importance of the ica locus for biofilm production and pathogenesis of S. epidermidis.
摘要
为了检测表皮葡萄球菌中生物膜的形成是否依赖于多糖细胞间黏附素（其生物合成由ica基因座驱动），将一个含有ica基因座的质粒转移到三株ica基因阴性菌株中。通过体外生物膜检测和大鼠中心静脉导管感染模型，我们证实了ica基因座对表皮葡萄球菌生物膜产生和致病机制的重要性。
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本文引用的文献
1
Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228).
Mol Microbiol. 2003 Sep;49(6):1577-93. doi: 10.1046/j.1365-2958.2003.03671.x.
2
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J Infect Dis. 2003 Sep 1;188(5):706-18. doi: 10.1086/377239. Epub 2003 Aug 11.
3
Lack of biofilm contribution to bacterial colonisation in an experimental model of foreign body infection by Staphylococcus aureus and Staphylococcus epidermidis.
FEMS Immunol Med Microbiol. 2003 Mar 20;35(2):135-40. doi: 10.1016/S0928-8244(02)00463-7.
4
Staphylococcus and biofilms.
Mol Microbiol. 2002 Mar;43(6):1367-78. doi: 10.1046/j.1365-2958.2002.02827.x.
5
In vivo models to evaluate adhesion and biofilm formation by Staphylococcus epidermidis.
Methods Enzymol. 2001;336:206-15. doi: 10.1016/s0076-6879(01)36591-6.
6
Characterization of the importance of Staphylococcus epidermidis autolysin and polysaccharide intercellular adhesin in the pathogenesis of intravascular catheter-associated infection in a rat model.
J Infect Dis. 2001 Apr 1;183(7):1038-42. doi: 10.1086/319279. Epub 2001 Mar 1.
7
Screening for Staphylococcus epidermidis markers discriminating between skin-flora strains and those responsible for infections of joint prostheses.
J Infect Dis. 2000 Jul;182(1):351-5. doi: 10.1086/315660. Epub 2000 Jul 6.
8
PCR-Based assay for discrimination between invasive and contaminating Staphylococcus epidermidis strains.
J Clin Microbiol. 2000 Feb;38(2):877-80. doi: 10.1128/JCM.38.2.877-880.2000.
9
Characterization of Staphylococcus epidermidis polysaccharide intercellular adhesin/hemagglutinin in the pathogenesis of intravascular catheter-associated infection in a rat model.
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10
Characterization of the importance of polysaccharide intercellular adhesin/hemagglutinin of Staphylococcus epidermidis in the pathogenesis of biomaterial-based infection in a mouse foreign body infection model.
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