Martins Katheryne Benini, Ferreira Adriano Martison, Pereira Valéria Cataneli, Pinheiro Luiza, de Oliveira Adilson, da Cunha Maria de Lourdes Ribeiro de Souza
Department of Microbiology and Immunology, Institute of Biosciences, UNESP - Universidade Estadual Paulista, Botucatu, Brazil.
Department of Tropical Diseases, Botucatu School of Medicine University Hospital, UNESP - Universidade Estadual Paulista, Botucatu, Brazil.
Front Microbiol. 2019 Jan 28;10:40. doi: 10.3389/fmicb.2019.00040. eCollection 2019.
Bacterial biofilms play an important role in urinary tract infections (UTIs), being responsible for persistent infections that lead to recurrences and relapses. is one of the main etiological agents of UTIs, however, little is known about biofilm production in this species and especially about its response to the antimicrobial agents used to treat UTIs when a biofilm is present. For this reason, the aim of this work was to evaluate the response of biofilms to five antimicrobial agents. was evaluated for antimicrobial susceptibility in its planktonic form by means of minimum inhibitory concentration (MIC) and in biofilms by means of minimum inhibitory concentration in biofilm (MICB) against the following antimicrobial agents by the microdilution technique: vancomycin, oxacillin, trimethoprim/sulfamethoxazole, ciprofloxacin, and norfloxacin. Of the 169 studied, 119 produced a biofilm as demonstrated by the polystyrene plate adherence method. Biofilm cells of exhibited a considerable increase in MICB when compared to the planktonic forms, with an increase of more than 32 times in the MICB of some drugs. Some isolates switched from the category of susceptible in the planktonic condition to resistant in the biofilm state. Statistical analysis of the results showed a significant increase in MICB ( < 0.0001) for all five drugs tested in the biofilm state compared to the planktonic form. Regarding determination of the minimum bactericidal concentration in biofilm (MBCB), there were isolates for which the minimum bactericidal concentration of all drugs was equal to or higher than the highest concentration tested.
细菌生物膜在尿路感染（UTIs）中起着重要作用，是导致持续性感染从而引起复发和再发的原因。 是UTIs的主要病原体之一，然而，对于该菌种生物膜的形成，尤其是其在存在生物膜时对用于治疗UTIs的抗菌药物的反应了解甚少。因此，本研究的目的是评估 生物膜对五种抗菌药物的反应。通过微量稀释技术，以最低抑菌浓度（MIC）评估其浮游形式的抗菌敏感性，并以生物膜最低抑菌浓度（MICB）评估生物膜状态下对以下抗菌药物的敏感性：万古霉素、苯唑西林、甲氧苄啶/磺胺甲恶唑、环丙沙星和诺氟沙星。在所研究的169株 中，通过聚苯乙烯平板黏附法证明有119株产生了生物膜。与浮游形式相比， 生物膜细胞的MICB显著增加，某些药物的MICB增加了32倍以上。一些菌株从浮游状态下的敏感类别转变为生物膜状态下的耐药类别。结果的统计分析表明，与浮游形式相比，在生物膜状态下测试的所有五种药物的MICB均显著增加（ < 0.0001）。关于生物膜最低杀菌浓度（MBCB）的测定，存在所有药物的最低杀菌浓度等于或高于测试最高浓度的菌株。
