红细胞黏附性/聚集性：一种用于检测具有动脉粥样硬化血栓形成危险因素和已确诊血管疾病个体低度体内炎症的新型生物标志物。

Erythrocyte adhesiveness/aggregation: a novel biomarker for the detection of low-grade internal inflammation in individuals with atherothrombotic risk factors and proven vascular disease.

作者信息

Berliner Shlomo, Rogowski Ori, Aharonov Stanislav, Mardi Tamar, Tolshinsky Tatiana, Rozenblat Meirav, Justo Dan, Deutsch Varda, Serov Jack, Shapira Itzhak, Zeltzer David

机构信息

Department of Internal Medicine D, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.

出版信息

Am Heart J. 2005 Feb;149(2):260-7. doi: 10.1016/j.ahj.2004.05.058.

DOI:10.1016/j.ahj.2004.05.058

PMID:15846263

Abstract

BACKGROUND

We have introduced a concept of using the erythrocyte as a sensor for the detection of enhanced inflammation-sensitive protein concentrations. We presently evaluated the capability of this new biomarker to detect the presence of inflammation in individuals with a history of a vascular disease as opposed to individuals with atherothrombotic risk factors but no clinically evident vascular disease.

METHODS

The degree of erythrocyte adhesiveness/aggregation was determined in the peripheral venous blood by using a simple blood test. Blood was drawn into a syringe containing sodium citrate and trickled onto a slide at an angle of 30 degrees. The slides were than scanned by a blinded technician by using an image analyzer to determine the area that is covered by the erythrocytes.

RESULTS

One hundred fifty-six subjects (61 women and 95 men) of 2586 (1238 women and 1348 men) met the criteria of a definite vascular disease (history of stroke, myocardial infarction, coronary artery bypass grafting, or peripheral vascular disease). The degree of erythrocyte aggregation was significantly (P = .008) higher in men, but not in women, with vascular disease as opposed to these without a vascular disease. The results of receiver operating characteristic curve analysis confirmed the diagnostic superiority of the erythrocyte aggregation biomarker over other commonly used markers of the acute phase in men. Similar results were obtained by using discriminant analysis. Finally, a significant correlation was found between the degree of erythrocyte aggregation and other markers of the acute phase suggesting its relevance for the detection and quantitation of low-grade inflammation in individuals with atherothrombosis.

CONCLUSION

Erythrocyte adhesiveness/aggregation may be a useful biomarker to detect internal inflammation in individuals with atherothrombosis.

摘要

背景

我们引入了一种利用红细胞作为传感器来检测炎症敏感蛋白浓度升高的概念。我们目前评估了这种新生物标志物在检测有血管疾病史个体中炎症存在的能力，与有动脉粥样硬化血栓形成风险因素但无临床明显血管疾病的个体进行对比。

方法

通过一项简单的血液检测来测定外周静脉血中红细胞黏附/聚集程度。将血液抽取到含有柠檬酸钠的注射器中，并以30度角滴到载玻片上。然后由一名不知情的技术人员使用图像分析仪扫描载玻片，以确定红细胞覆盖的面积。

结果

2586名受试者（1238名女性和1348名男性）中有156名（61名女性和95名男性）符合明确血管疾病（中风、心肌梗死、冠状动脉搭桥术或外周血管疾病史）的标准。与无血管疾病的人相比，患有血管疾病的男性红细胞聚集程度显著更高（P = 0.008），但女性并非如此。受试者工作特征曲线分析结果证实，红细胞聚集生物标志物在男性中诊断优于其他常用的急性期标志物。通过判别分析也获得了类似结果。最后，发现红细胞聚集程度与急性期的其他标志物之间存在显著相关性，表明其与动脉粥样硬化血栓形成个体中低度炎症的检测和定量相关。