Fiege N, Klatte D, Kollmann D, Zahner H, Bürger H J
Institut für Parasitologie der Justus-Liebig-Universität, Giessen, Federal Republic of Germany.
Parasitol Res. 1992;78(1):32-8. doi: 10.1007/BF00936178.
IgM, IgG1, and IgG2 antibodies to Eimeria bovis first-generation merozoite antigens were determined by enzyme-linked immunosorbent assay and Western blotting in naturally infected cows and in their offspring before and after the uptake of colostrum. In addition, calves were examined following experimental primary and challenge infections. Neonate calves received maternal antibodies via colostrum. All isotypes determined were transmitted, but only IgG1 was concentrated in the colostrum and it occurred at significantly increased levels in sera from the calves as compared with those from the respective dams. Recognition patterns (Western blotting) displayed by related maternal serum and colostrum and those shown by calves that had ingested colostrum were very similar, but marked variations occurred between individual pairs. Experimental infection of 15-week-old calves with 0.7 x 10(5) oocysts caused strong protective immunity against a challenge with 1 x 10(5) oocysts. In contrast, animals that had undergone a weak intercurrent infection were not protected. Experimental infections induced a considerable increase in IgG1 and IgG2 antibody levels, whereas IgM values increased only slightly. The spectrum of merozoite antigens recognized by the sera increased markedly after experimental infection, although high individual variations were found in the calves. However, there was no correlation between the levels of any specific antibody or the recognition patterns and the status of immunity to a severe challenge.
通过酶联免疫吸附测定和蛋白质印迹法，测定了自然感染的奶牛及其初乳摄入前后的后代中针对牛艾美耳球虫第一代裂殖子抗原的IgM、IgG1和IgG2抗体。此外，对实验性初次感染和攻毒感染后的犊牛进行了检查。新生犊牛通过初乳获得母源抗体。所测定的所有同种型均能传递，但只有IgG1在初乳中浓缩，并且与各自母畜的血清相比，犊牛血清中IgG1水平显著升高。相关母源血清和初乳所显示的识别模式（蛋白质印迹法）与摄入初乳的犊牛所显示的模式非常相似，但个体之间存在明显差异。用0.7×10⁵个卵囊对15周龄犊牛进行实验性感染，可对1×10⁵个卵囊的攻毒产生强大的保护性免疫。相比之下，经历过轻度并发感染的动物没有受到保护。实验性感染导致IgG1和IgG2抗体水平显著升高，而IgM值仅略有增加。实验性感染后，血清识别的裂殖子抗原谱明显增加，尽管犊牛中存在高度个体差异。然而，任何特异性抗体的水平或识别模式与对严重攻毒的免疫状态之间均无相关性。
