Rose M E, Peppard J V, Hobbs S M
Parasite Immunol. 1984 Jan;6(1):1-12. doi: 10.1111/j.1365-3024.1984.tb00777.x.
The antibody responses of rats to infection with the intestinal intracellular protozoan parasite Eimeria nieschulzi were examined by a sensitive radio-immunoassay with a soluble preparation of sporulated oocysts as antigen. Specific antibodies of the IgM, IgG1, IgG2a and IgG2b isotypes were found in the blood circulation and IgA antibodies were detected in the bile and in intestinal washings. The IgM response was rapid, its peak was relatively brief and it was not recalled by the reinoculation of oocysts. There were some differences between the responses in the different subclasses of IgG but they all reached a peak between 20-30 days after the initiation of the primary infection and there was an anamnestic response to a challenge inoculation of oocysts. IgA antibodies to E. nieschulzi antigen in the bile and in intestinal washings increased and decreased after both primary and secondary inocula. Antibodies of all isotypes tested were virtually absent in the blood circulation of infected athymic rats. These findings are discussed with reference to antibody responses to other parasitic infections and to the role of antibodies in immunity to coccidiosis.
通过以孢子化卵囊的可溶性制剂作为抗原的灵敏放射免疫测定法，检测了大鼠对肠道细胞内原生动物寄生虫尼氏艾美耳球虫感染的抗体反应。在血液循环中发现了IgM、IgG1、IgG2a和IgG2b同种型的特异性抗体，并且在胆汁和肠道冲洗液中检测到了IgA抗体。IgM反应迅速，其峰值相对短暂，再次接种卵囊不会使其重现。IgG不同亚类的反应存在一些差异，但它们在初次感染开始后的20至30天之间均达到峰值，并且对卵囊的攻击接种有回忆反应。初次和二次接种后，胆汁和肠道冲洗液中针对尼氏艾美耳球虫抗原的IgA抗体均先增加后减少。在感染的无胸腺大鼠的血液循环中，几乎不存在所有测试同种型的抗体。结合对其他寄生虫感染的抗体反应以及抗体在抗球虫病免疫中的作用，对这些发现进行了讨论。
