Buchta Christoph, Bittner Claudia, Heinzl Harald, Höcker Paul, Macher Maria, Mayerhofer Matthias, Schmid Rainer, Seger Christoph, Dettke Markus
Department of Blood Group Serology and Transfusion Medicine, the Institute for Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria.
Transfusion. 2005 May;45(5):798-802. doi: 10.1111/j.1537-2995.2005.04380.x.
Di(2-ethylhexyl)phthalate (DEHP) is a plasticizer that can leach from medical devices including storage bags for plateletpheresis concentrates (PCs). In this study, the DEHP exposure to patients receiving PCs was determined and several variables were evaluated to reduce DEHP load to PC recipients.
In 12 patients, serum DEHP was assessed before and after PC transfusion. For in vitro investigations, PCs were produced either with donor plasma or with 65 percent additive solution (AS; T-Sol) and stored for 5 days. Washing of PCs was performed according to AABB standards. DEHP levels were determined by gas chromatography-mass spectrometry.
Transfusion of PCs led to a significant increase in serum DEHP. DEHP levels in the PCs continuously increased during storage, although the accumulation of DEHP was less in PCs stored in the AS, T-Sol, than when stored in plasma. Storage-related accumulation of DEHP contributed to a major part of the total DEHP load in PCs stored for 5 days. Washing of PCs led to a reduction of DEHP load.
Recipients of PCs are exposed to DEHP, although the total amount represents only a small percentage of the defined tolerable intake. Reduction of storage time, the storage of PC in T-Sol, or the exchange of the storage medium before transfusion are practicable means to reduce the DEHP load in PC.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种增塑剂，可从包括血小板单采浓缩物（PC）储存袋在内的医疗设备中渗出。在本研究中，测定了接受PC治疗的患者的DEHP暴露情况，并评估了几个变量以降低PC接受者的DEHP负荷。
对12名患者在输注PC前后的血清DEHP进行评估。在体外研究中，PC分别用供体血浆或65%添加剂溶液（AS；T-Sol）制备，并储存5天。PC的洗涤按照美国血库协会（AABB）标准进行。通过气相色谱-质谱法测定DEHP水平。
输注PC导致血清DEHP显著增加。PC中的DEHP水平在储存期间持续升高，尽管储存在AS、T-Sol中的PC中DEHP的积累比储存在血浆中的少。与储存相关的DEHP积累占储存5天的PC中总DEHP负荷的主要部分。洗涤PC可降低DEHP负荷。
PC接受者会接触到DEHP，尽管总量仅占规定可耐受摄入量的一小部分。减少储存时间、将PC储存在T-Sol中或在输血前更换储存介质是降低PC中DEHP负荷的可行方法。
