de Las Heras Alarcón Carolina, Twaites Beverley, Cunliffe David, Smith James R, Alexander Cameron
School of Pharmacy and Biomedical Science, University of Portsmouth, White Swan Road, Portsmouth PO1 2DT, UK.
Int J Pharm. 2005 May 13;295(1-2):77-91. doi: 10.1016/j.ijpharm.2005.01.037.
A series of responsive polymers displaying pH and temperature-mediated phase changes were prepared from N-isopropylacrylamide and omega-carboxylic acid functionalised acrylamides. These polymers were grafted to surfaces and their characteristics probed by atomic force microscopy in aqueous solutions. The effects of pH and temperature induced phase transitions on the short-term adsorption of the bacteria Salmonella typhimurium and Bacillus cereus from pure cultures were assessed. Contact angle studies indicated that pH and temperature-dependent surface properties were exhibited by the graft polymer surfaces. Temperature-dependent surface morphology changes occurred through polymer graft phase transitions as observed in AFM and accompanying changes in adhesion forces underwater were found to correlate with surface properties obtained from contact angle measurements. Adsorption of S. typhimurium and B. cereus was not significantly altered as a function of pH, but attachment of both bacterial strains increased at temperatures above the polymer coil-globule transition indicating the importance of switching surface hydrophobicity in controlling short-term bacterial adsorption.
一系列具有pH值和温度介导相变的响应性聚合物由N-异丙基丙烯酰胺和ω-羧酸官能化丙烯酰胺制备而成。这些聚合物被接枝到表面，并通过原子力显微镜在水溶液中对其特性进行探测。评估了pH值和温度诱导的相变对鼠伤寒沙门氏菌和蜡样芽孢杆菌纯培养物短期吸附的影响。接触角研究表明，接枝聚合物表面呈现出pH值和温度依赖性的表面性质。通过原子力显微镜观察到，随着聚合物接枝相变，发生了温度依赖性的表面形态变化，并且发现水下粘附力的伴随变化与通过接触角测量获得的表面性质相关。鼠伤寒沙门氏菌和蜡样芽孢杆菌的吸附并没有随着pH值的变化而显著改变，但在高于聚合物线圈-球状体转变温度时，两种细菌菌株的附着均增加，这表明在控制细菌短期吸附中切换表面疏水性的重要性。
