Alslev Bo, Korsgaard Bodil, Bjerregaard Poul
Institute of Biology, University of Southern Denmark, Odense, Campusvej 55, DK-5230 Odense M, Denmark.
Aquat Toxicol. 2005 May 15;72(4):295-304. doi: 10.1016/j.aquatox.2005.01.005.
The estrogenic effect of butylparaben was investigated in a rainbow trout Oncorhynchus mykiss test system. Butylparaben was administered orally to sexually immature rainbow trout every second day for up to 10 days in doses between 4 and 74 mgkg(-1)2d(-1) and in the water at 35 and 201 microgl(-1) for 12 days. Plasma vitellogenin was measured before and during the exposures and the concentrations of butylparaben in liver and muscle were determined at the end of experiments. Increases in average plasma vitellogenin levels were seen at oral exposure to 9 mg butylparaben kg(-1)2d(-1). The ED50 values for increase in vitellogenin synthesis were 46, 29 and 10.5 mg butylparaben kg(-1)2d(-1), respectively, at day 3, 6 and 12. Exposure to 201 microg butylparaben l(-1) increased vitellogenin synthesis, but exposure to 35 microgl(-1) did not. Butylparaben showed little tendency to bioaccumulation in rainbow trout; less than 1 per thousand of the total amount of butylparaben administered orally at 51 mgkg(-1)2d(-1) over the 12 days experimental period was retained in liver at the end of the experiment. After 12 days exposure to 35 and 201 microg butylparaben l(-1), plasma concentrations were 9 and 183 microgl(-1), respectively, and for the fish exposed to 201 microgl(-1) there was a positive correlation between concentrations of vitellogenin and butylparaben in the plasma. On the assumption that butylparaben removed from the water phase during water exposure were taken up into the fish, butylparaben uptake rates in the fish exposed to 35 and 201 microg butylparaben l(-1) were 13 and 78 mgkg(-1)day(-1), respectively.
在虹鳟鱼（Oncorhynchus mykiss）测试系统中研究了对羟基苯甲酸丁酯的雌激素效应。每隔一天给未成熟的虹鳟鱼口服对羟基苯甲酸丁酯，持续10天，剂量为4至74毫克/千克体重/两天，同时在水中以35和201微克/升的浓度暴露12天。在暴露前和暴露期间测量血浆卵黄蛋白原，并在实验结束时测定肝脏和肌肉中对羟基苯甲酸丁酯的浓度。口服9毫克对羟基苯甲酸丁酯/千克体重/两天时，平均血浆卵黄蛋白原水平升高。在第3天、第6天和第12天，卵黄蛋白原合成增加的半数有效剂量（ED50）值分别为46、29和10.5毫克对羟基苯甲酸丁酯/千克体重/两天。暴露于201微克/升对羟基苯甲酸丁酯会增加卵黄蛋白原的合成，但暴露于35微克/升则不会。对羟基苯甲酸丁酯在虹鳟鱼中几乎没有生物累积的趋势；在为期12天的实验期内，以51毫克/千克体重/两天的剂量口服对羟基苯甲酸丁酯，实验结束时肝脏中保留的对羟基苯甲酸丁酯总量不到千分之一。在暴露于35和201微克/升对羟基苯甲酸丁酯12天后，血浆浓度分别为9和183微克/升，对于暴露于201微克/升的鱼，血浆中卵黄蛋白原浓度与对羟基苯甲酸丁酯浓度之间存在正相关。假设在水暴露期间从水相中去除的对羟基苯甲酸丁酯被鱼吸收，暴露于35和201微克/升对羟基苯甲酸丁酯的鱼的摄取率分别为13和78毫克/千克体重/天。
