Murugesan P, Senthilkumar J, Balasubramanian K, Aruldhas M M, Arunakaran J
Department of Endocrinology, Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India.
Hum Exp Toxicol. 2005 Feb;24(2):61-6. doi: 10.1191/0960327105ht500oa.
To clarify the reproductive toxicity of polychlorinated biphenyl compounds through determination of testicular lipid peroxidation, reactive oxygen species and enzymatic and non-enzymatic antioxidants in rats exposed to Aroclor 1254. Adult male rats were administered Aroclor 1254 at a dose of 2 mg/kg per day ip for 30 days. The rats were sacrificed 24 hours after last dosing and the serum and other tissues collected and processed for relevant determinations. The body weight and the weights of the testis, epididymis, ventral prostate and seminal vesicle and the serum testosterone and estradiol were significantly decreased in Aroclor 1254 treated rats. The testicular lipid peroxidation, hydrogen peroxide and hydroxyl radical were significantly elevated whereas, testicular antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and glutathione reductase (GR) were significantly decreased. The non-enzymatic antioxidants, vitamin C and vitamin E, were also decreased. These results suggest that Aroclor 1254 induces an increase in the lipid peroxidation, hydrogen peroxide and hydroxyl radical and diminish in the antioxidant defense system in rats, indicating that the free radical-dependent mechanism may play an important role in the testicular toxicity of polychlorinated biphenyls.
通过测定暴露于Aroclor 1254的大鼠睾丸脂质过氧化、活性氧以及酶促和非酶促抗氧化剂，以阐明多氯联苯化合物的生殖毒性。成年雄性大鼠每天腹腔注射2 mg/kg剂量的Aroclor 1254，持续30天。在末次给药24小时后处死大鼠，收集血清和其他组织并进行相关测定。Aroclor 1254处理的大鼠体重、睾丸、附睾、腹侧前列腺和精囊重量以及血清睾酮和雌二醇水平显著降低。睾丸脂质过氧化、过氧化氢和羟自由基显著升高，而睾丸抗氧化酶，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）、谷胱甘肽-S-转移酶（GST）和谷胱甘肽还原酶（GR）显著降低。非酶促抗氧化剂维生素C和维生素E也减少。这些结果表明，Aroclor 1254可诱导大鼠脂质过氧化、过氧化氢和羟自由基增加，抗氧化防御系统减弱，表明自由基依赖性机制可能在多氯联苯的睾丸毒性中起重要作用。
