Zhu Ai Ping, Chan-Park Mary B
Biological and Chemical Processing Laboratory, School of Mechanical and Production Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Republic of Singapore.
J Biomater Sci Polym Ed. 2005;16(3):301-16. doi: 10.1163/1568562053654149.
Chitosan-modified biodegradable hydrogels were prepared by UV irradiation of solutions in mild aqueous acidic media of poly(caprolactone)-co-poly(ethylene glycol)-co-poly(caprolactone) diacrylate (PCL-PEG-PCL-DA) and chitosan. Hydrogels obtained were characterized using FT-IR, DSC, TGA and XPS. FT-IR, TGA and DSC revealed the semi-interpenetrating polymer network structure formed in the hydrogel. Though the water swelling degree of these chitosan-modified hydrogels was substantial in the range of 322-539%, it was found that fibroblasts could still attach, spread and grow on them; this is in contrast to the commonly investigated PEG-diacrylate hydrogel. The MTT assay demonstrated that cells could grow better on 3 or 6% chitosan-modified hydrogel than unmodified PCL-PEG-PCL-DA hydrogels or low-content (1%) chitosan-modified PCL-PEG-PCL-DA hydrogel. Increased chitosan content resulted in increased cell interaction and also decreased water swelling, both of which results in increased cell attachment and spread.
壳聚糖改性的可生物降解水凝胶是通过在聚（己内酯）-共-聚（乙二醇）-共-聚（己内酯）二丙烯酸酯（PCL-PEG-PCL-DA）和壳聚糖的温和水性酸性介质溶液中进行紫外线照射制备的。使用傅里叶变换红外光谱（FT-IR）、差示扫描量热法（DSC）、热重分析法（TGA）和X射线光电子能谱（XPS）对所得水凝胶进行表征。FT-IR、TGA和DSC揭示了水凝胶中形成的半互穿聚合物网络结构。尽管这些壳聚糖改性水凝胶的水溶胀度在322-539%范围内很大，但发现成纤维细胞仍能在其上附着、铺展和生长；这与常用的聚乙二醇二丙烯酸酯水凝胶形成对比。MTT法表明，细胞在3%或6%壳聚糖改性水凝胶上比未改性的PCL-PEG-PCL-DA水凝胶或低含量（1%）壳聚糖改性的PCL-PEG-PCL-DA水凝胶上生长得更好。壳聚糖含量的增加导致细胞相互作用增加，同时水溶胀度降低，这两个结果都导致细胞附着和铺展增加。
