靶点可及性决定了人类RNA诱导沉默复合体（RISC）的效力。
Target accessibility dictates the potency of human RISC.
作者信息
Brown Kirk M, Chu Chia-Ying, Rana Tariq M
机构信息
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
出版信息
Nat Struct Mol Biol. 2005 May;12(5):469-70. doi: 10.1038/nsmb931. Epub 2005 Apr 24.
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC()) catalysis. Kinetic studies revealed that siRNA-programmed RISC() cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.
在本报告中，我们研究了增加靶位点可及性对活化的人RNA诱导沉默复合体（RISC()）催化作用的影响。动力学研究表明，当靶位点可及性增加时，由小干扰RNA（siRNA）编程的RISC()能更高效地切割靶RNA。这些结果证明靶位点可及性与RISC(*)催化作用相关。
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