Motoi Yurie, Inoue Satoshi, Hatta Hajime, Sato Kozue, Morimoto Kinjiro, Yamada Akio
The United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan.
Jpn J Infect Dis. 2005 Apr;58(2):115-8.
We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemistry, indicating that IgY to rabies recombinant proteins could serve as a reagent for diagnosis of rabies virus infection.
我们通过用在大肠杆菌中表达的狂犬病病毒（CVS-11株）的重组His标签核蛋白和磷蛋白（rN、rP）免疫母鸡，获得了狂犬病特异性蛋黄抗体（IgY）。通过蛋白质印迹法、免疫荧光测定法和免疫组织化学法表明，抗rN和rP IgY能特异性结合狂犬病病毒CVS-11株的相应蛋白，这表明针对狂犬病重组蛋白的IgY可作为诊断狂犬病病毒感染的试剂。
