Penney Lisa, Smith Anderson, Coates Brent, Wijewickreme Arosha
CANTEST Ltd.-Food Residue, 4606 Canada Way, Burnaby, British Columbia V5G IK5, Canada.
J AOAC Int. 2005 Mar-Apr;88(2):645-53.
A new liquid chromatography/mass spectrometry (LC/MS) method is presented for the determination of chloramphenicol (CAP) residues in milk, eggs, chicken muscle and liver, and beef muscle and kidney. CAP is extracted from the samples with acetonitrile and defatted with hexane. The acetonitrile extracts are then evaporated, and residues are reconstituted in 10mM ammonium acetate--acetonitrile mobile phase and injected into the LC system. CAP is determined by reversed-phase chromatography using an Inertsil ODS-2 column and MS detection with negative ion electrospray ionization. Calibration curves were linear between 0.5-5.0 ng/g for all matrixes studied. The relative standard deviations for measurements by this method were generally <12%, and average recoveries ranged from 80 to 120%, depending on the matrix involved. The method detection limits of CAP ranged from 0.2 to 0.6 ng/g, which are comparable to previously reported results. The proposed method is rapid, simple, and specific, allowing a single analyst to easily prepare over 40 samples in a regular working day.
本文介绍了一种用于测定牛奶、鸡蛋、鸡肉肌肉和肝脏以及牛肉肌肉和肾脏中氯霉素（CAP）残留量的新型液相色谱/质谱（LC/MS）方法。CAP用乙腈从样品中提取，并用己烷脱脂。然后将乙腈提取物蒸发，残留物用10mM醋酸铵 - 乙腈流动相复溶并注入LC系统。采用Inertsil ODS - 2柱通过反相色谱法测定CAP，并采用负离子电喷雾电离进行质谱检测。在所研究的所有基质中，校准曲线在0.5 - 5.0 ng/g之间呈线性。该方法测量的相对标准偏差通常<12%，平均回收率根据所涉及的基质在80%至120%之间。CAP的方法检测限为0.2至0.6 ng/g，与先前报道的结果相当。所提出的方法快速、简单且具有特异性，一名分析师在正常工作日内可以轻松制备40多个样品。
