Matsui Sei-Ichi, LaDuca Jeffrey, Rossi Michael R, Nowak Norma J, Cowell John K
Roswell Park Cancer Institute, Department of Cancer Genetics, Buffalo, NY 14263, USA.
Cancer Genet Cytogenet. 2005 May;159(1):18-26. doi: 10.1016/j.cancergencyto.2004.09.010.
Spectral karyotyping of prostate cell lines LNCaP, DU145, PC3, and 22RV demonstrated structural chromosome rearrangements involving the distal long arm of chromosome 4. In all but 22RV, these are nonreciprocal translocations between chromosomes 4 and 10. In 22RV, an apparently reciprocal t(2q;4q) is seen. Fluorescence in situ hybridization analysis of the chromosome 4 translocation breakpoints demonstrated that deletions were associated with all of the translocations, resulting in a net loss of chromosome material. Overlapping deletions in 4q28 approximately 34 were seen in LNCap, DU145, and 22RV, which defined an approximately 4.5-megabase pair common region of deletion. The deletion in PC3 was more proximal on 4q, involving the 4q21 approximately q26 region. A meta analysis of high-resolution definition of losses of chromosome material from published studies demonstrates that loss of 4q material may occur in at least 50% of primary tumors. This analysis defines a series of genes in the critical 4q region, which is potentially associated with prostate tumor development.
对前列腺癌细胞系LNCaP、DU145、PC3和22RV进行光谱核型分析，结果显示存在涉及4号染色体长臂远端的染色体结构重排。除22RV外，在其他细胞系中，这些都是4号染色体和10号染色体之间的非相互易位。在22RV中，可见明显的相互易位t(2q;4q)。对4号染色体易位断点进行荧光原位杂交分析表明，所有易位均伴有缺失，导致染色体物质净损失。在LNCap、DU145和22RV中可见4q28约34处的重叠缺失，确定了一个约4.5兆碱基对的共同缺失区域。PC3中的缺失在4q上更靠近近端，涉及4q21约q26区域。对已发表研究中染色体物质缺失的高分辨率定义进行的荟萃分析表明，至少50%的原发性肿瘤可能发生4q物质缺失。该分析确定了关键4q区域中的一系列基因，这些基因可能与前列腺肿瘤的发生发展相关。
