Bolton Sally, Butt Arthur M
Centre for Neuroscience Research, Hodgkin Building, GKT Guy's Campus, King's College, London Bridge, London, SE1 1UL, UK.
J Pharmacol Toxicol Methods. 2005 May-Jun;51(3):221-33. doi: 10.1016/j.vascn.2004.08.010.
The rodent optic nerve is a model tissue for the physiological investigation of axonal-glial interactions in a typical CNS white matter tract. There is strong evidence that nerve transmission is maintained by a considerable degree of dynamic signalling between axons and glia through a variety of mechanisms, such as regulation of the ionic environment, energy metabolism and calcium signalling. This review focuses on the methods used to examine axonal and glial functions and interactions, primarily in the rodent optic nerve. Techniques encompass intracellular microelectrodes, sucrose- and grease-gap recordings of membrane potentials, suction electrode to measure compound action potentials, the use of ion-sensitive electrodes, patch clamping and imaging. An overview of the advantages and drawbacks of each technique is given and the application of each to the understanding glial and axonal physiology is briefly discussed.
啮齿动物的视神经是一种典型的中枢神经系统白质束中轴突与神经胶质细胞相互作用生理研究的模型组织。有充分证据表明，轴突和神经胶质细胞之间通过多种机制，如离子环境调节、能量代谢和钙信号传导，进行相当程度的动态信号传递来维持神经传导。本综述主要聚焦于用于研究轴突和神经胶质细胞功能及相互作用的方法，主要是在啮齿动物的视神经中。技术包括细胞内微电极、蔗糖和油隙膜电位记录、用于测量复合动作电位的吸液电极、离子敏感电极的使用、膜片钳和成像。本文给出了每种技术的优缺点概述，并简要讨论了每种技术在理解神经胶质细胞和轴突生理学方面的应用。
