Konnerth A, Orkand R K
Neurosci Lett. 1986 May 6;66(1):49-54. doi: 10.1016/0304-3940(86)90164-3.
Changes in dye absorption and fluorescence produced by electrical stimulation were measured in frog optic nerves stained with voltage-sensitive dyes. Following a single maximal stimulus applied through a suction electrode, the change in transmitted light intensity consisted of two components: one representing an axonal compound action potential and the second a slow depolarizing afterpotential which appeared to arise from the glial cells. The following results support this interpretation: during a train of stimuli the depolarizing potentials sum and can exceed 80% of the initial spike amplitude while the spike amplitude itself remains essentially constant. Thus, the axons cannot have undergone significant depolarization during the train. Optical recordings with simultaneous microelectrode recordings from the glial cells indicate that the change in glial membrane potential during the train has a time-course similar to that of the slow optical response. We conclude that voltage-sensitive dyes can monitor potential changes in both neurons and glia.
在用电压敏感染料染色的青蛙视神经中，测量了电刺激引起的染料吸收和荧光变化。通过吸电极施加单次最大刺激后，透射光强度的变化由两个部分组成：一个代表轴突复合动作电位，另一个是缓慢的去极化后电位，似乎起源于神经胶质细胞。以下结果支持这一解释：在一串刺激期间，去极化电位总和可超过初始动作电位幅度的80%，而动作电位幅度本身基本保持恒定。因此，在这串刺激期间轴突不可能发生显著去极化。同时从神经胶质细胞进行微电极记录的光学记录表明，这串刺激期间神经胶质细胞膜电位的变化与缓慢光学反应具有相似的时间进程。我们得出结论，电压敏感染料可以监测神经元和神经胶质细胞中的电位变化。
