Leo Cornelia, Richter Christine, Horn Lars-Christian, Schütz Alexander, Pilch Henryk, Höckel Michael
Department of Gynecology, Leipzig University, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany.
Gynecol Oncol. 2005 May;97(2):602-6. doi: 10.1016/j.ygyno.2005.01.044.
The aim of this study was to investigate the expression of the proapoptotic protein Apaf-1 in cervical cancers. Moreover, we studied its correlation to intratumoral pO(2) and to clinico-pathological parameters.
86 patients with cervical cancer were subjected to intratumoral pO(2) measurement with the Eppendorf electrode. From these patients, cervical cancer tissue was used for immunohistochemistry with an anti-Apaf-1 antibody.
Apaf-1 is expressed in cervical cancer. Cervical cancers with strong or moderate Apaf-1 expression had significantly less lymph node metastases at time of surgery than tumors with weak or negative Apaf-1 expression (P = 0.022). There was no significant correlation between Apaf-1 expression and intratumoral pO(2), pT stage, FIGO stage, lymphovascular space involvement, and grade.
Loss of Apaf-1 expression may represent a marker of aggressive tumor behavior since it correlates significantly with the occurrence of lymph node metastasis in cervical cancer.
本研究旨在调查促凋亡蛋白Apaf-1在宫颈癌中的表达情况。此外，我们还研究了其与肿瘤内pO₂以及临床病理参数的相关性。
86例宫颈癌患者使用Eppendorf电极进行肿瘤内pO₂测量。从这些患者中，取宫颈癌组织用抗Apaf-1抗体进行免疫组织化学检测。
Apaf-1在宫颈癌中表达。Apaf-1表达强或中度的宫颈癌在手术时的淋巴结转移明显少于Apaf-1表达弱或阴性的肿瘤（P = 0.022）。Apaf-1表达与肿瘤内pO₂、pT分期、国际妇产科联盟（FIGO）分期、脉管间隙浸润及分级之间无显著相关性。
Apaf-1表达缺失可能代表侵袭性肿瘤行为的一个标志物，因为它与宫颈癌淋巴结转移的发生显著相关。
