[某种物质]表达下调与淋巴结转移相关，并预示IB期宫颈鳞状细胞癌预后不良：一项病例对照研究

Down-regulated expression of is associated with lymph node metastasis and predicts poor outcome in stage IB cervical squamous cell carcinoma: a case-control study.

作者信息

Zhang Gongyi, Zhang Rong, Bai Ping, Li Shumin, Zuo Jing, Zhang Yuanyuan, Liu Mei, Wu Lingying

机构信息

Department of Gynecologic Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Laboratory of Cell and Molecular Biology and State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

出版信息

Ann Transl Med. 2022 Jun;10(12):663. doi: 10.21037/atm-22-2483.

DOI:10.21037/atm-22-2483

PMID:35845501

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9279763/

Abstract

BACKGROUND

Lymph node metastasis (LNM) accounts for the most important route of metastasis for cervical cancer. Yet, the status of LNM is different in patients with similar clinico-pathological variables. It has been revealed that microRNAs are widely involved in the occurrence and development of various malignancies, and the tumor-suppressive or promoting effects of () family have been previously reported. This study sought to investigate the predictive value of for lymphogenous spread and its effect on the survival of patients with early-stage cervical squamous cell cancer (CSCC).

METHODS

Patients with stage IB squamous cervical cancer who were treated surgically between October 2015 and November 2018 were enrolled. A total of 21 formalin-fixed paraffin-embedded tissues of pathologically confirmed positive lymph nodes were retrieved, and an additional 21 tissues of negative lymph nodes from patients well-matched on baseline characteristics were collected as the control group. TaqMan real-time quantitative polymerase chain reaction was used to examine the expression levels of in the samples. Differential expression levels of were compared between the 2 groups using independent sample t-test. Furthermore, the associations between expression level and clinico-pathological parameters of these 42 patients was evaluated by Chi-square test or Fisher's exact-probability method, and their effects on survival were assessed using Kaplan-Meier product-limit method.

RESULTS

There were no significant differences in baseline clinico-pathological parameters between the 2 groups (P>0.05). The expression levels of in the node-positive group and control group were 1.61±3.09 and 16.77±30.40, respectively (P=0.029). Downregulated expression of was closely related to depth of invasion (DOI) and lymph-vascular space invasion (P<0.05). Univariate analysis revealed that downregulated and deeper DOI were associated with worse 5-year disease-free survival, while multivariate analysis showed that only the expression level of was an independent factor for disease-free survival (HR =0.120; 95% CI: 0.015-0.979; P=0.048). Patients with downregulated tended to have more unfavorable overall survival, but the difference did not reach statistical significance.

CONCLUSIONS

plays an inhibitory role in the pathogenesis of lymph node metastasis and may serve as a novel prognostic biomarker for patients with CSCC.

摘要

背景

淋巴结转移（LNM）是宫颈癌最重要的转移途径。然而，在具有相似临床病理变量的患者中，LNM的情况有所不同。研究表明，微小RNA广泛参与各种恶性肿瘤的发生和发展，并且先前已经报道了（）家族的肿瘤抑制或促进作用。本研究旨在探讨（）对淋巴源性扩散的预测价值及其对早期宫颈鳞状细胞癌（CSCC）患者生存的影响。

方法

纳入2015年10月至2018年11月期间接受手术治疗的IB期宫颈鳞癌患者。共获取21份经病理证实为阳性淋巴结的福尔马林固定石蜡包埋组织，并收集另外21份来自基线特征匹配良好的患者的阴性淋巴结组织作为对照组。采用TaqMan实时定量聚合酶链反应检测样本中（）的表达水平。使用独立样本t检验比较两组之间（）的差异表达水平。此外，采用卡方检验或Fisher精确概率法评估这42例患者（）表达水平与临床病理参数之间的关联，并使用Kaplan-Meier乘积限界法评估其对生存的影响。

结果

两组之间的基线临床病理参数无显著差异（P>0.05）。淋巴结阳性组和对照组中（）的表达水平分别为1.61±3.09和16.77±30.40（P=0.029）。（）表达下调与浸润深度（DOI）和淋巴血管间隙浸润密切相关（P<0.05）。单因素分析显示，（）表达下调和更深的DOI与较差的5年无病生存率相关，而多因素分析表明，只有（）的表达水平是无病生存的独立因素（HR =0.120；95%CI：0.015-0.979；P=0.048）。（）表达下调的患者总体生存往往更差，但差异未达到统计学意义。