英国牛支原体田间分离株的插入序列分析

Insertion sequence profiling of UK Mycoplasma bovis field isolates.

作者信息

Miles Katie, McAuliffe Laura, Persson Anja, Ayling Roger D, Nicholas Robin A J

机构信息

Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.

出版信息

Vet Microbiol. 2005 May 20;107(3-4):301-6. doi: 10.1016/j.vetmic.2005.02.003. Epub 2005 Mar 17.

DOI:10.1016/j.vetmic.2005.02.003

PMID:15863291

Abstract

The presence and distribution of insertion sequences ISMbov2 and ISMbov3 within Mycoplasma bovis were investigated. Analysis was carried out by Southern blotting using specific probes of 221 bp and 185 bp, to detect ISMbov2 and ISMbov3, respectively, amplified from the homologous sequences ISMmy1 and IS1634 within Mycoplasma mycoides subspecies mycoides small colony type. We present data obtained from 49 field isolates of M. bovis, originating from pneumonic lungs, collected within the United Kingdom between 1996 and 2002. Hybridisation profiles show considerable variation between strains. ISMbov2 sequences are present between 2 and 17 copies while there are between 3 and 14 copies of the IS1634 homologue ISMbov3. These data also provide support for previous analysis by random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).

摘要

研究了牛支原体中插入序列ISMbov2和ISMbov3的存在情况及分布。通过Southern杂交进行分析，分别使用从蕈状支原体亚种蕈状支原体小菌落型内的同源序列ISMmy1和IS1634扩增得到的221 bp和185 bp特异性探针来检测ISMbov2和ISMbov3。我们展示了从1996年至2002年在英国收集的49株源自肺炎肺组织的牛支原体田间分离株获得的数据。杂交图谱显示菌株间存在相当大的差异。ISMbov2序列的拷贝数在2至17个之间，而IS1634同源物ISMbov3的拷贝数在3至14个之间。这些数据也为先前通过随机扩增多态性DNA（RAPD）和扩增片段长度多态性（AFLP）进行的分析提供了支持。