McAuliffe Laura, Kokotovic Branko, Ayling Roger D, Nicholas Robin A J
Mycoplasma Group, Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency (Weybridge), Surrey, United Kingdom.
J Clin Microbiol. 2004 Oct;42(10):4556-65. doi: 10.1128/JCM.42.10.4556-4565.2004.
Mycoplasma bovis is an important veterinary pathogen causing pneumonia, arthritis, and mastitis in infected cattle. We investigated the genetic diversity of 53 isolates collected in the United Kingdom between 1996 and 2002 with pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), and random amplified polymorphic DNA (RAPD) analysis. In addition, the influence of variable surface protein (Vsp) profiles on the profiles generated with molecular typing techniques was studied. Both AFLP and RAPD separated the isolates into two distinct groups, but PFGE showed less congruence with the other techniques. There was no clear relationship between the geographic origin or year of isolation of the isolates and the profiles produced. No correlation between Vsp profiles and any of the molecular typing techniques was observed. We propose that RAPD and AFLP provide valuable tools for molecular typing of M. bovis.
牛支原体是一种重要的兽医病原体,可导致感染牛的肺炎、关节炎和乳腺炎。我们使用脉冲场凝胶电泳(PFGE)、扩增片段长度多态性(AFLP)和随机扩增多态性DNA(RAPD)分析,对1996年至2002年间在英国收集的53株分离株的遗传多样性进行了研究。此外,还研究了可变表面蛋白(Vsp)谱对分子分型技术产生的谱型的影响。AFLP和RAPD都将分离株分为两个不同的组,但PFGE与其他技术的一致性较差。分离株的地理来源或分离年份与产生的谱型之间没有明显的关系。未观察到Vsp谱与任何分子分型技术之间存在相关性。我们认为,RAPD和AFLP为牛支原体的分子分型提供了有价值的工具。