Blizard David A, Wada Yumiko, Onuki Yasuhiro, Kato Katsunori, Mori Toshiyuki, Taniuchi Tohru, Hosokawa Hiroshi, Otobe Takayuki, Takahashi Aki, Shisa Hayase, Hiai Hiroshi, Makino Junshiro
Center for Developmental and Health Genetics, Pennsylvania State University, University Park, Pennsylvania 16801, USA.
Behav Genet. 2005 May;35(3):323-32. doi: 10.1007/s10519-005-3224-1.
The present paper evaluates the inclusion of a standard strain or outbred stock in multi-strain behavioral phenotyping protocols to perform the same role as the external standard in biochemical assay procedures. As potential standards, the F344 inbred strain and an outbred stock of Long Evans were tested with three other inbred strains. To evaluate the influence of rearing conditions on phenotype stability, one group of F344s was born at the University of Tsukuba, another, bred elsewhere and delivered to Tsukuba at 4 weeks of age. All animals were tested in open-field (OF), runway emergence (RE) and digging tests as adults. The results showed no influence of breeding or transportation history on OF and RE behavior of the two F344 groups, while there was evidence that digging behavior may be affected by the different rearing experience. The inclusion of a 'standard strain or stock' in phenotyping protocols involving multiple inbred strains or lines of rats, mice and flies has obvious advantages by providing a reference point for inter-laboratory comparisons. The properties of inbred strains and outbred stocks favorable to their use as standards are discussed.
本文评估了在多品系行为表型分析实验方案中纳入标准品系或远交种群，使其发挥与生化分析程序中的外部标准相同的作用。作为潜在标准，F344近交系和Long Evans远交种群与其他三个近交系一起进行了测试。为了评估饲养条件对表型稳定性的影响，一组F344在筑波大学出生，另一组在其他地方繁殖，并在4周龄时送到筑波。所有动物成年后均在旷场试验（OF）、跑道出现试验（RE）和挖掘试验中进行测试。结果表明，两个F344组的繁殖或运输历史对OF和RE行为均无影响，而有证据表明挖掘行为可能受不同饲养经历的影响。在涉及大鼠、小鼠和果蝇的多个近交系或品系的表型分析实验方案中纳入“标准品系或种群”，通过为实验室间比较提供参考点具有明显优势。本文还讨论了有利于将近交系和远交种群用作标准的特性。
