Voigt Birger, Kuramoto Takashi, Mashimo Tomoji, Tsurumi Toshiko, Sasaki Yoshiyuki, Hokao Ryoji, Serikawa Tadao
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto.
Physiol Genomics. 2008 Feb 19;32(3):335-42. doi: 10.1152/physiolgenomics.00158.2007. Epub 2007 Nov 27.
Recombinant inbred (RI) strains are formed from an outcross between two well-characterized inbred stains followed by at least 20 generations of inbreeding. RI strains can be utilized for the analysis of many complex phenotypic traits. The LEXF/FXLE RI strain set consists of 34 RI strains derived by reciprocal crossing of LE/Stm and F344/Stm. Here we report on genetic dissections of complex traits using this RI set and their parental strains. We have developed strain distribution patterns for 232 informative simple sequence length polymorphism markers. The framework map covers the rat genome except for chromosome Y. Seventy-six phenotype parameters, which included physiological and behavioral traits, were examined for these RI lines. Quantitative trait locus (QTL) analysis of these parameters revealed 27 significant and 91 suggestive QTLs, illustrating the potential of this RI resource for the detection of underlying gene functions for various phenotypes. Although this RI set was originally developed to study susceptibility to chemical-induced tumors, it has been shown to be equally powerful for a wide spectrum of traits. The LEXF/FXLE RI strains have been deposited at the National Bio Resource Project for the Rat in Japan and are maintained under specific pathogen-free conditions. They are available at http://www.anim.med.kyoto-u.ac.jp/nbr.
重组近交（RI）系由两个特征明确的近交品系杂交后再经过至少20代的近亲繁殖形成。RI系可用于分析许多复杂的表型性状。LEXF/FXLE RI系由LE/Stm和F344/Stm相互杂交产生的34个RI系组成。在此，我们报告使用该RI系及其亲本品系对复杂性状进行的遗传剖析。我们已经为232个信息丰富的简单序列长度多态性标记开发了品系分布模式。该框架图谱覆盖了除Y染色体外的大鼠基因组。对这些RI系检测了76个表型参数，包括生理和行为性状。对这些参数的数量性状位点（QTL）分析揭示了27个显著QTL和91个暗示性QTL，说明了该RI资源在检测各种表型潜在基因功能方面的潜力。尽管该RI系最初是为研究化学诱导肿瘤的易感性而开发的，但已证明它在广泛的性状研究中同样有效。LEXF/FXLE RI系已保存在日本大鼠国家生物资源项目中，并在无特定病原体条件下维持。可通过http://www.anim.med.kyoto-u.ac.jp/nbr获取。
