Troubacheeva N V, Salina E A, Pershina L A
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Genetika. 2005 Mar;41(3):349-55.
Using RELP analysis with three probes homologous to specific regions of mitochondrial DNA genes and PCR analysis of the mitochondrial recombining-repeat-sequence 18S/5S region of cereals, five alloplasmic wheat lines of different origin and fertility expression were studied. These lines are self-pollinated progeny of BC1-BC4 generations of barley-wheat hybrids Hordeum geniculatum All. (2n = 28) x Triticum aestivum L. (2n = 42). It was found that recombinant alloplasmic lines characterized by partial fertility contain either maternal (barley) DNA fragments or maternal and paternal (wheat) DNA fragments simultaneously (heteroplasmy). In lines with stable expression of self-fertility, fragments of only paternal mitochondrial DNA were detected. It is assumed that in alloplasmic lines, there is the interrelation between the presence of definite fragments of the mitochondrial genome belonging to either parental type and fertility expression.
利用与线粒体DNA基因特定区域同源的三种探针进行RELP分析，并对谷物线粒体重组重复序列18S/5S区域进行PCR分析，研究了五个不同来源和育性表达的异质小麦系。这些品系是大麦-小麦杂种Hordeum geniculatum All.（2n = 28）×普通小麦Triticum aestivum L.（2n = 42）的BC1 - BC4代自花授粉后代。研究发现，部分可育的重组异质系要么同时含有母本（大麦）DNA片段，要么同时含有母本和父本（小麦）DNA片段（异质性）。在自交育性稳定表达的品系中，仅检测到父本线粒体DNA片段。据推测，在异质系中，属于亲本类型的线粒体基因组特定片段的存在与育性表达之间存在相互关系。
