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[通过 46 条染色体的大麦 - 小麦 BC1 杂种海大麦草亚种古斯索纳海大麦(= 膝曲大麦 H. geniculatum All.)(2n = 28)× 普通小麦(2n = 42)自花授粉形成可育整倍体系(2n = 42)的特征]

[Features of the formation of self-fertile euploid lines (2n = 42) by self-pollination of the 46-chromosome barley-wheat BC1 hybrid Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) x Triticum aestivum L. (2n = 42)].

作者信息

Pershina L A, Trubacheeva N V, Rakovtseva T S, Belova L I, Deviatkina E P, Kravtsova L A

出版信息

Genetika. 2006 Dec;42(12):1683-90.

Abstract

We studied some features of the development of self-fertile 42-chromosome lines on the base of self-pollination progeny of 46-chromosome plants obtained by backcrossing of barley--wheat hybrids Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) x Triticum aestivum L. (2n = 42). The stabilization of karyotypes, resulting in 42-chromosome plants of the wheat type was generally completed by generation BC1F10. The plants of all self-pollination progenies, including BC1F10, showed some phenotypic traits characteristic of wild barley. Plants of BC1F10 with the chromosome sets 2n = 42 and 2n = 42 + t were analyzed by RAPD with a set of 115 primers. Fragments of the wild barley genome were detected in RAPD patterns with 19 primers. Cross-hybridization confirmed that these fragments belonged to the wild barley genome. We raised four phenotypically different 42-chromosome lines from grains obtained from plants of generation BC1F10, and these lines proved to be cytogenetically stable and self-fertile when grown in the field.

摘要

我们以大麦-小麦杂种(海滨大麦亚种古斯通大麦哈德逊(= 膝曲大麦奥尔)(2n = 28)×普通小麦L.(2n = 42))回交得到的46条染色体植株的自花授粉后代为基础,研究了可自育的42条染色体品系的一些发育特征。核型的稳定,从而产生小麦类型的42条染色体植株,一般在回交一代10代(BC1F10)时完成。所有自花授粉后代的植株,包括BC1F10,都表现出一些野生大麦特有的表型特征。用一组115个引物对具有2n = 42和2n = 42 + t染色体组的BC1F10植株进行了随机扩增多态性DNA(RAPD)分析。用19个引物在RAPD图谱中检测到了野生大麦基因组的片段。杂交证实这些片段属于野生大麦基因组。我们从BC1F10代植株收获的种子中培育出了四个表型不同的42条染色体品系,这些品系在田间种植时经细胞遗传学鉴定是稳定的且可自育。

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