Irons Robert, Pinge-Filho Phileno, Fritsche Kevin L
Department of Nutritional Sciences, Division of Animal Sciences, University of Missouri, Columbia 65211, USA.
J Nutr. 2005 May;135(5):1151-6. doi: 10.1093/jn/135.5.1151.
We previously reported that in a mouse model, a diet high in (n-3) PUFA diminishes host survival following an infection from Listeria monocytogenes, a gram-positive bacterial pathogen. In this study we investigated the impact of (n-3) PUFA on the adaptive immune response to L. monocytogenes. BALB/c mice were fed experimental diets either devoid of or rich in (n-3) PUFA from fish oil for 4 wk and then infected with 10(6) actA-deficient L. monocytogenes. At 7 and 35 d postchallenge, effector and memory/effector T cells in the spleen were enumerated by flow cytometry. Surprisingly, the number of Listeria-specific CD4(+) and CD8(+) effector and memory/effector T cells in the spleen was not affected by (n-3) PUFA. Also, the effector cells derived from mice fed either diet were equally capable of conferring protective immunity upon adoptive transfer to naive recipients. Despite our previous data, which demonstrated that (n-3) PUFA profoundly impaired host resistance to L. monocytogenes, pathogen-specific T cell responses were not substantially affected by dietary (n-3) PUFA.
我们之前报道过，在一个小鼠模型中，富含（n-3）多不饱和脂肪酸（PUFA）的饮食会降低宿主在感染单核细胞增生李斯特菌（一种革兰氏阳性细菌病原体）后的存活率。在本研究中，我们调查了（n-3）PUFA对针对单核细胞增生李斯特菌的适应性免疫反应的影响。将BALB/c小鼠用不含或富含来自鱼油的（n-3）PUFA的实验性饮食喂养4周，然后用10^6个缺乏actA的单核细胞增生李斯特菌进行感染。在攻击后第7天和第35天，通过流式细胞术对脾脏中的效应T细胞和记忆/效应T细胞进行计数。令人惊讶的是，脾脏中李斯特菌特异性CD4(+)和CD8(+)效应T细胞以及记忆/效应T细胞的数量不受（n-3）PUFA的影响。此外，来自喂食任何一种饮食的小鼠的效应细胞在过继转移到未感染的受体时，赋予保护性免疫的能力是相同的。尽管我们之前的数据表明（n-3）PUFA严重损害了宿主对单核细胞增生李斯特菌的抵抗力，但病原体特异性T细胞反应并未受到饮食中（n-3）PUFA的实质性影响。
