Faucette K J, Parker C J, McCluskey T, Bernshaw N J, Rodgers G M
Department of Pediatrics, University of Utah Medical Center, Salt Lake City.
Blood. 1992 Jun 1;79(11):2888-95.
Molecules that induce tissue factor expression by responsive cells such as endothelial cells and monocytes may be important in the regulation of hemostasis and, perhaps, in mediating certain hemostatic disorders. A constituent of normal human plasma capable of inducing tissue factor activity in human endothelial cells and monocytes has been isolated and identified as a derivative of, or modification associated with albumin. Procoagulant albumin caused a concentration-dependent induction of tissue factor expression by human endothelial cells, but bovine endothelial cells were unresponsive. The dose-response curve developed a plateau phase, indicating that the capacity of endothelial cells to respond to the stimulus was finite. The maximum response induced by the procoagulant albumin was similar to that observed for maximally effective concentrations of endotoxin, interleukin-1, and tumor necrosis factor. Time-course studies showed that procoagulant albumin produced peak activity in 4 to 6 hours. Identification of a procoagulant form of albumin in normal human plasma suggests a potential role for this constituent in regulation of hemostasis.
能够诱导内皮细胞和单核细胞等反应性细胞表达组织因子的分子，可能在止血调节中起重要作用，或许还参与介导某些止血障碍。一种能够诱导人内皮细胞和单核细胞产生组织因子活性的正常人血浆成分已被分离出来，并被鉴定为白蛋白的衍生物或与之相关的修饰物。促凝白蛋白可引起人内皮细胞组织因子表达呈浓度依赖性诱导，但牛内皮细胞无反应。剂量反应曲线出现平台期，表明内皮细胞对该刺激的反应能力是有限的。促凝白蛋白诱导的最大反应与内毒素、白细胞介素 -1 和肿瘤坏死因子最大有效浓度时观察到的反应相似。时间进程研究表明，促凝白蛋白在 4 至 6 小时内产生峰值活性。在正常人血浆中鉴定出促凝形式的白蛋白，提示该成分在止血调节中可能具有潜在作用。
