Cormack B P, Struhl K
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
Cell. 1992 May 15;69(4):685-96. doi: 10.1016/0092-8674(92)90232-2.
Using temperature- and proteolytically sensitive derivatives to inactivate the function of the yeast TATA-binding protein (TBP) in vivo, we investigated the requirement of TBP for transcription by the three nuclear RNA polymerases in yeast cells. TBP is required for RNA polymerase II (pol II) transcription from promoters containing conventional TATA elements as well as functionally distinct promoters that lack TATA-like sequences. TBP is also required for transcription of the U6 snRNA and two different tRNA genes mediated by RNA pol III as well as transcription of ribosomal RNA mediated by RNA pol I. For all promoters tested, transcription decreases rapidly and specifically upon inactivation of TBP, strongly suggesting that TBP is directly involved in the transcription process. These observations suggest that TBP is required for transcription of all nuclearly encoded genes in yeast, although distinct molecular mechanisms are probably involved for the three RNA polymerase transcription machineries.
利用对温度和蛋白水解敏感的衍生物在体内使酵母TATA结合蛋白（TBP）的功能失活，我们研究了酵母细胞中三种核RNA聚合酶转录时TBP的需求情况。对于含有传统TATA元件的启动子以及缺乏TATA样序列但功能不同的启动子，RNA聚合酶II（pol II）转录都需要TBP。U6 snRNA转录以及由RNA pol III介导的两个不同tRNA基因的转录，还有由RNA pol I介导的核糖体RNA转录也都需要TBP。对于所有测试的启动子，TBP失活后转录会迅速且特异性地降低，这强烈表明TBP直接参与转录过程。这些观察结果表明，酵母中所有核编码基因的转录都需要TBP，尽管三种RNA聚合酶转录机制可能涉及不同的分子机制。
