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静止期酿酒酵母细胞中封闭的 35S 核糖体 RNA 基因染色质的建立。

Establishment of closed 35S ribosomal RNA gene chromatin in stationary Saccharomyces cerevisiae cells.

机构信息

Regensburg Center of Biochemistry (RCB), Institut für Biochemie, Genetik und Mikrobiologie, Universität Regensburg, Lehrstühle Biochemie III und Genetik, Universitätsstr. 31, 93053 Regensburg, Germany.

出版信息

Nucleic Acids Res. 2024 Nov 11;52(20):12208-12226. doi: 10.1093/nar/gkae838.

DOI:10.1093/nar/gkae838
PMID:39373531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11551728/
Abstract

As a first step in eukaryotic ribosome biogenesis RNA polymerase (Pol) I synthesizes a large ribosomal RNA (rRNA) precursor from multicopy rRNA gene loci. This process is essential for cellular growth and regulated in response to the cell's physiological state. rRNA gene transcription is downregulated upon growth to stationary phase in the yeast Saccharomyces cerevisiae. This reduction correlates with characteristic changes in rRNA gene chromatin structure from a transcriptionally active 'open' state to a non-transcribed 'closed' state. The conserved lysine deacetylase Rpd3 was shown to be required for this chromatin transition. We found that Rpd3 is needed for tight repression of Pol I transcription upon growth to stationary phase as a prerequisite for the establishment of the closed chromatin state. We provide evidence that Rpd3 regulates Pol I transcription by adjusting cellular levels of the Pol I preinitiation complex component core factor (CF). Importantly, our study identifies CF as the complex limiting the number of open rRNA genes in exponentially growing and stationary cells.

摘要

在真核生物核糖体生物发生过程中，RNA 聚合酶（Pol）I 从多拷贝 rRNA 基因座合成一个大的核糖体 RNA（rRNA）前体。这个过程对细胞生长至关重要，并根据细胞的生理状态进行调节。在酵母酿酒酵母中，当生长到静止期时，rRNA 基因转录会下调。这种减少与 rRNA 基因染色质结构从转录活跃的“开放”状态到非转录的“关闭”状态的特征变化相关。已证明保守的赖氨酸去乙酰化酶 Rpd3 对于这种染色质转变是必需的。我们发现，Rpd3 在生长到静止期时，对于 Pol I 转录的紧密抑制是必需的，这是建立封闭染色质状态的前提。我们提供的证据表明，Rpd3 通过调节 Pol I 转录起始复合物成分核心因子（CF）的细胞水平来调节 Pol I 转录。重要的是，我们的研究确定了 CF 是限制指数生长期和静止期细胞中开放 rRNA 基因数量的复合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/433df2457d56/gkae838fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/b860a771c766/gkae838figgra1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/4fbe698b044b/gkae838fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/d7d5a3f03313/gkae838fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/77417043be87/gkae838fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/ff800c2d90bb/gkae838fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/f05c98d45a6d/gkae838fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/6094325bd0c5/gkae838fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/433df2457d56/gkae838fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/b860a771c766/gkae838figgra1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/4fbe698b044b/gkae838fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/d7d5a3f03313/gkae838fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/77417043be87/gkae838fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/ff800c2d90bb/gkae838fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/f05c98d45a6d/gkae838fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/6094325bd0c5/gkae838fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9468/11551728/433df2457d56/gkae838fig7.jpg

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2
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3
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Chromosome Res. 2019 Mar;27(1-2):31-40. doi: 10.1007/s10577-018-09603-9. Epub 2019 Jan 8.
4
Co-repressor, co-activator and general transcription factor: the many faces of the Sin3 histone deacetylase (HDAC) complex.共抑制因子、共激活因子和一般转录因子：Sin3 组蛋白去乙酰化酶 (HDAC) 复合物的多面性。
Biochem J. 2018 Dec 14;475(24):3921-3932. doi: 10.1042/BCJ20170314.
5
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6
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7
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