Gök Uzeyir, Alpay Hayrettin Cengiz, Akpolat Nusret, Yoldaş Tahir, Kilic Abdullah, Yilmaz Bayram, Kabakuş Nimet
Firat University, Medical Faculty, Otorhinolaryngology Department, Elaziğ, Turkey.
Int J Pediatr Otorhinolaryngol. 2005 Sep;69(9):1199-204. doi: 10.1016/j.ijporl.2005.03.025.
To induce experimental peripheral facial paralysis by inoculation of HSV1 and to compare the effects of steroid, acyclovir, lipoprostoglandin E2 and steroid + acyclovir treatments in terms of clinical recovery, electrophysiologically and histopathologically.
A total of 132 adult female rats were used in this study. HSV type 1 strain was inoculated at the back of the left ear by using 27 gauge needle. Of all animals, 70 (53%) rats which developed facial paralysis were divided into five groups (n = 14 for each group) as control, steroid + acyclovir, lipoprostaglandin E1, steroid only and acyclovir only. At the end of the 21 days period, the rats were clinically examined and electrophysiological tests were performed, then decapitated and the nerve specimens were obtained.
A modified electroneurography (ENoG) test was performed and the latencies and the amplitudes were compared. The findings of the intact side were better, but with no significant difference. Histopathologicaly edema was significantly smaller in all groups compared to the controls (p < 0.05). Similarly, no difference was seen in terms of vacuolar degeneration and Schwann cell hyperchromatisation among the groups and no significant difference in recovery period and rate of facial paralysis when all groups were compared.
Facial paralysis induced by HSV1 recovered spontaneously within a week. In the treatment of facial paralysis, steroid alone, acyclovir alone, steroid + acyclovir, or lipoprostaglandin E1 all reduced edema in the infected facial nerve but there was no statistical difference in of the rate or degree of recovery.
通过接种单纯疱疹病毒1型（HSV1）诱导实验性周围性面瘫，并在临床恢复、电生理和组织病理学方面比较类固醇、阿昔洛韦、脂前列腺素E2以及类固醇+阿昔洛韦治疗的效果。
本研究共使用132只成年雌性大鼠。用27号针头将1型单纯疱疹病毒株接种于左耳后。在所有动物中，70只（53%）出现面瘫的大鼠被分为五组（每组n = 14），即对照组、类固醇+阿昔洛韦组、脂前列腺素E1组、仅用类固醇组和仅用阿昔洛韦组。在21天结束时，对大鼠进行临床检查并进行电生理测试，然后断头并获取神经标本。
进行了改良的神经电图（ENoG）测试并比较了潜伏期和波幅。健侧的结果更好，但无显著差异。组织病理学上，与对照组相比，所有组中的水肿均显著减轻（p < 0.05）。同样，各组之间在空泡变性和施万细胞色素沉着方面未见差异，并且在比较所有组时，面瘫的恢复期和恢复率也无显著差异。
HSV1诱导的面瘫在一周内自发恢复。在面瘫治疗中，单独使用类固醇、单独使用阿昔洛韦、类固醇+阿昔洛韦或脂前列腺素E1均能减轻感染面神经的水肿，但恢复率或恢复程度无统计学差异。
