Todorov Svetoslav D, Wachsman Mónica B, Knoetze Hendriëtte, Meincken Martina, Dicks Leon M T
Department of Microbiology, Stellenbosch University, 7600 Stellenbosch, South Africa.
Int J Antimicrob Agents. 2005 Jun;25(6):508-13. doi: 10.1016/j.ijantimicag.2005.02.005.
Enterococcus mundtii ST4V, isolated from soya beans, produces a 3950Da antibacterial peptide active against Gram-positive and Gram-negative bacteria, including Enterococcus faecalis, Streptococcus spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae and Staphylococcus aureus. The peptide also inactivated the herpes simplex viruses HSV-1 (strain F) and HSV-2 (strain G), a polio virus (PV3, strain Sabin) and a measles virus (strain MV/BRAZIL/001/91, an attenuated strain of MV). MV, HSV-1 and HSV-2 were 95.5%-99.9% inactivated by peptide ST4V at 400 microg/ml. Monkey kidney Vero cells were not inactivated, even at four times the level peptide ST4V displayed antiviral activity, indicating that the effect was not due to cytotoxicity. Complete inactivation or significant reduction in antimicrobial activity was observed after treatment of peptide ST4V with Proteinase K, pronase, pepsin and trypsin. No change in antimicrobial activity was recorded after treatment with alpha-amylase, suggesting that peptide ST4V was not glycosylated. This is the first description of an antibacterial and antiviral peptide with such broad-spectrum of activity, produced by a lactic acid bacterium.
从大豆中分离出的蒙氏肠球菌ST4V可产生一种3950Da的抗菌肽，该肽对革兰氏阳性菌和革兰氏阴性菌均有活性，包括粪肠球菌、链球菌属、铜绿假单胞菌、肺炎克雷伯菌、肺炎链球菌和金黄色葡萄球菌。该肽还可使单纯疱疹病毒HSV-1（F株）和HSV-2（G株）、脊髓灰质炎病毒（PV3，Sabin株）以及麻疹病毒（MV/BRAZIL/001/91株，一种减毒麻疹病毒株）失活。在400μg/ml的浓度下，肽ST4V可使MV、HSV-1和HSV-2的活性降低95.5%-99.9%。即使在肽ST4V显示抗病毒活性的四倍浓度下，猴肾Vero细胞也未被灭活，这表明该效应并非由细胞毒性引起。用蛋白酶K、链霉蛋白酶、胃蛋白酶和胰蛋白酶处理肽ST4V后，观察到其抗菌活性完全丧失或显著降低。用α-淀粉酶处理后，抗菌活性未发生变化，这表明肽ST4V未被糖基化。这是首次描述由乳酸菌产生的具有如此广谱活性的抗菌和抗病毒肽。
