Cannon J G, Bott K F
Mol Gen Genet. 1979 Jul 13;174(2):149-62. doi: 10.1007/BF00268352.
Spectinomycin-resistant mutants of Bacillus subtilis show three different types of alterations in sporulation ability. Class 1 mutants can both grow and sporulate in the presence of spectinomycin. Class 2 mutants can grow in the presence of spectinomycin, but are unable to sporulate in either the presence or absence of spectinomycin. Class 3 mutants have a conditional phenotype, and are able to sporulate in the absence of spectinomycin, but not in its presence. The ability of these strains to produce alkaline phosphatase, a biochemical marker for early sporulation events, is correlated with the ability to sporulate in the presence or absence of antibiotic. All of the spectinomycin-resistance mutations could be genetically linked to the cysA marker, and a mutational alteration of a protein of the 30S ribosomal subunit has been identified in one of the Class 3 strains (Spc 1-11). Fine-structure mapping of the spectinomycin resistance mutation of strain Spc 1-11 confirmed its location in the cluster of genes for ribosomal components on the B. subtilis genetic map. Genetic analysis indicated that the properties of the Class 1 and Class 2 mutants result from more than one mutation. The spectinomycin-resistance and altered sporulation properties of the two Class 3 mutants probably result from a single genetic lesion.
枯草芽孢杆菌的壮观霉素抗性突变体在芽孢形成能力上表现出三种不同类型的改变。1类突变体在壮观霉素存在的情况下既能生长又能形成芽孢。2类突变体在壮观霉素存在的情况下能够生长，但在有或没有壮观霉素的情况下都不能形成芽孢。3类突变体具有条件性表型，在没有壮观霉素的情况下能够形成芽孢，但在其存在时则不能。这些菌株产生碱性磷酸酶（一种早期芽孢形成事件的生化标志物）的能力与在有或没有抗生素的情况下形成芽孢的能力相关。所有壮观霉素抗性突变都可以在遗传上与cysA标记相连，并且在其中一个3类菌株（Spc 1-11）中鉴定出了30S核糖体亚基一种蛋白质的突变改变。对菌株Spc 1-11的壮观霉素抗性突变进行的精细结构定位证实了其在枯草芽孢杆菌遗传图谱上核糖体成分基因簇中的位置。遗传分析表明，1类和2类突变体的特性是由多个突变导致的。两个3类突变体的壮观霉素抗性和改变的芽孢形成特性可能是由单个遗传损伤引起的。
