Hill S H
J Gen Microbiol. 1983 Feb;129(2):293-302. doi: 10.1099/00221287-129-2-293.
Three sporulation mutants have been isolated which produce spores with an atypical resistance phenotype, i.e. they are sensitive to organic solvents and heat but resistant to lysozyme. All three mutants produced serine protease, alkaline phosphatase and glucose dehydrogenase which are biochemical marker events for stages I, II and III. Two of the three mutants produced dipicolinic acid, a late marker, but the third was defective in its production. Heat-resistance was not restored to any of the mutants by the provision of exogenous dipicolinate. Gel electrophoresis showed that the mutant spores had similar patterns of spore coat proteins to the wild-type and electron microscopy revealed no significant structural differences. The three mutations responsible for the phenotypes of the mutant spores lie in the phe-argA region of the Bacillus subtilis chromosome. Recombination index values indicate that the mutations are in three separate genes. They define at least two new sporulation loci, designated spoVH and spoVJ.
已分离出三个芽孢形成突变体，它们产生的芽孢具有非典型的抗性表型，即对有机溶剂和热敏感，但对溶菌酶有抗性。所有这三个突变体都产生了丝氨酸蛋白酶、碱性磷酸酶和葡萄糖脱氢酶，这些是芽孢形成第一、二和三阶段的生化标记事件。三个突变体中的两个产生了吡啶二羧酸，这是一个后期标记，但第三个突变体在其产生方面存在缺陷。通过提供外源吡啶二羧酸，没有任何一个突变体恢复耐热性。凝胶电泳显示，突变体芽孢的芽孢衣蛋白模式与野生型相似，电子显微镜检查未发现明显的结构差异。导致突变体芽孢表型的三个突变位于枯草芽孢杆菌染色体的phe-argA区域。重组指数值表明这些突变存在于三个不同的基因中。它们定义了至少两个新的芽孢形成位点，命名为spoVH和spoVJ。
