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通过甲臜还原后的荧光原位杂交技术在6小时内对牛奶中呼吸型假单胞菌属进行计数。

Enumeration of respiring Pseudomonas spp. in milk within 6 hours by fluorescence in situ hybridization following formazan reduction.

作者信息

Kitaguchi Akiko, Yamaguchi Nobuyasu, Nasu Masao

机构信息

Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.

出版信息

Appl Environ Microbiol. 2005 May;71(5):2748-52. doi: 10.1128/AEM.71.5.2748-2752.2005.

DOI:10.1128/AEM.71.5.2748-2752.2005
PMID:15870367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1087560/
Abstract

Respiring Pseudomonas spp. in milk were quantified within 6 h by fluorescence in situ hybridization (FISH) with vital staining. FISH with an oligonucleotide probe based on 16S rRNA sequences was used for the specific detection of Pseudomonas spp. at the single cell level. 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was used to estimate bacterial respiratory activity. The numbers of respiring Pseudomonas cells as determined by FISH with CTC staining (CTC-FISH) were almost the same or higher than the numbers of CFU as determined by the conventional culture method.

摘要

通过荧光原位杂交（FISH）结合活体染色，在6小时内对牛奶中呼吸型假单胞菌属进行了定量分析。基于16S rRNA序列的寡核苷酸探针的FISH用于在单细胞水平上特异性检测假单胞菌属。使用5-氰基-2,3-二苯基氯化四氮唑（CTC）来估计细菌的呼吸活性。通过CTC染色的FISH（CTC-FISH）测定的呼吸型假单胞菌细胞数量与传统培养方法测定的菌落形成单位（CFU）数量几乎相同或更高。

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本文引用的文献

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Simplified sample preparation using frame spotting method for direct counting of total bacteria by fluorescence microscopy.使用载玻片点样法简化样本制备，通过荧光显微镜直接计数总细菌数。
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4
Seven-hour fluorescence in situ hybridization technique for enumeration of Enterobacteriaceae in food and environmental water sample.用于食品和环境水样中肠杆菌科细菌计数的七小时荧光原位杂交技术
J Appl Microbiol. 2003;95(6):1182-90. doi: 10.1046/j.1365-2672.2003.02051.x.
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