Torisawa Yu-Suke, Takagi Airi, Shiku Hitoshi, Yasukawa Tomoyuki, Matsue Tomokazu
Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
Oncol Rep. 2005 Jun;13(6):1107-12.
The respiratory activity of a multicellular spheroid was non-invasively monitored by scanning electrochemical microscopy (SECM) to investigate the anticancer drug sensitivity. The effects of the three anticancer drugs, cisplatin (CDDP), 5-fluorouracil (5-FU), and paclitaxel (TXL), were continuously evaluated based on respiratory activity for 5 days. The drug sensitivities obtained by SECM were higher compared to those evaluated by the spheroid volume and conformed to those evaluated by a conventional colorimetric assay. Our results show that the SECM-based assay directly correlates with the number of viable cells within the spheroid, whereas the spheroid volume does not necessarily correlate with the number of viable cells. Furthermore, the results obtained by spheroid culture (3-D) were compared to those of cells cultured in a flask (2-D) and within a collagen gel (3-D). The drug sensitivity of cells cultured in 2-D is more pronounced than that of the cells cultured in 3-D. Since the cellular proliferation status in a 3-D culture is similar to that in vivo, the drug sensitivity test performed in the spheroid culture will give meaningful results that can be extended to an in vivo application. A SECM-based assay is perfectly suitable to directly evaluate the drug sensitivity of the spheroid.
通过扫描电化学显微镜（SECM）对多细胞球体的呼吸活性进行无创监测，以研究抗癌药物敏感性。基于呼吸活性持续5天评估了三种抗癌药物顺铂（CDDP）、5-氟尿嘧啶（5-FU）和紫杉醇（TXL）的效果。与通过球体体积评估的药物敏感性相比，SECM获得的药物敏感性更高，且与通过传统比色法评估的结果相符。我们的结果表明，基于SECM的检测与球体内活细胞数量直接相关，而球体体积不一定与活细胞数量相关。此外，将球体培养（三维）获得的结果与在培养瓶中培养（二维）以及在胶原凝胶中培养（三维）的细胞的结果进行了比较。二维培养的细胞的药物敏感性比三维培养的细胞更明显。由于三维培养中的细胞增殖状态与体内相似，在球体培养中进行的药物敏感性测试将给出有意义的结果，可扩展到体内应用。基于SECM的检测非常适合直接评估球体的药物敏感性。
