Hasannejad Farkhonde, Montazeri Leila, Mano João F, Bonakdar Shahin, Fazilat Ahmad
Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, Semnan University of Medical Science, Semnan, Iran.
Genetic Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran.
Bioimpacts. 2024;14(3):29945. doi: 10.34172/bi.2023.29945. Epub 2023 Nov 28.
Cell culture-based technologies are widely utilized in various domains such as drug evaluation, toxicity assessment, vaccine and biopharmaceutical development, reproductive technology, and regenerative medicine. It has been demonstrated that pre-adsorption of extracellular matrix (ECM) proteins including collagen, laminin and fibronectin provide more degrees of support for cell adhesion. The purpose of cell imprinting is to imitate the natural topography of cell membranes by gels or polymers to create a reliable environment for the regulation of cell function. The results of recent studies show that cell imprinting is a tool to guide the behavior of cultured cells by controlling their adhesive interactions with surfaces. Therefore, in this review we aim to compare different cell cultures with the imprinting method and discuss different cell imprinting applications in regenerative medicine, personalized medicine, disease modeling, and cell therapy.
基于细胞培养的技术广泛应用于药物评估、毒性评估、疫苗和生物制药开发、生殖技术以及再生医学等各个领域。已经证明，预先吸附包括胶原蛋白、层粘连蛋白和纤连蛋白在内的细胞外基质（ECM）蛋白可为细胞黏附提供更多程度的支持。细胞印迹的目的是通过凝胶或聚合物模仿细胞膜的自然拓扑结构，以创建一个可靠的环境来调节细胞功能。最近的研究结果表明，细胞印迹是一种通过控制培养细胞与表面的黏附相互作用来指导其行为的工具。因此，在本综述中，我们旨在将不同的细胞培养方法与印迹方法进行比较，并讨论细胞印迹在再生医学、个性化医学、疾病建模和细胞治疗中的不同应用。
